Protective effects of Korean red ginseng against radiation-induced apoptosis in human HaCaT keratinocytes
| Autor: | Chul-Ho Kim, Yoo Seob Shin, Jae Won Chang, Hye Sook Hwang, Keun Hyung Park, Young-Taek Oh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Keratinocytes
Cell Survival Health Toxicology and Mutagenesis p38 mitogen-activated protein kinases Panax Radiation-Protective Agents Biology medicine.disease_cause Radiation Tolerance Cell Line Ginseng Cell Movement medicine Humans Radiology Nuclear Medicine and imaging radiation-induced mucositis Viability assay radioprotection Korean red ginseng (KRG) head and neck cancer radiation-inducedmucositis apoptosis Cell Proliferation chemistry.chemical_classification Reactive oxygen species Radiation Korea Dose-Response Relationship Drug Cell growth Plant Extracts Dose-Response Relationship Radiation HaCaT Oxidative Stress chemistry Apoptosis Immunology Cancer research Reactive Oxygen Species Oxidative stress |
| Zdroj: | Journal of Radiation Research JOURNAL OF RADIATION RESEARCH(55): 2 |
| ISSN: | 1349-9157 0449-3060 |
| Popis: | Radiation-induced oral mucositis is a dose-limiting toxic side effect for patients with head and neck cancer. Numerous attempts at improving radiation-induced oral mucositis have not produced a qualified treatment. Ginseng polysaccharide has multiple immunoprotective effects. Our aim was to investigate the effectiveness of Korean red ginseng (KRG) on radiation-induced damage in the human keratinocyte cell line HaCaT and in an in vivo zebrafish model. Radiation inhibited HaCaT cell proliferation and migration in a cell viability assay and wound healing assay, respectively. KRG protected against these effects. KRG attenuated the radiation-induced embryotoxicity in the zebrafish model. Irradiation of HaCaT cells caused apoptosis and changes in mitochondrial membrane potential (MMP). KRG inhibited the radiation-induced apoptosis and intracellular generation of reactive oxygen species (ROS), and stabilized the radiation-induced loss of MMP. Western blots revealed KRG-mediated reduced expression of ataxia telangiectasia mutated protein (ATM), p53, c-Jun N-terminal kinase (JNK), p38 and cleaved caspase-3, compared with their significant increase after radiation treatment. The collective results suggest that KRG protects HaCaT cells by blocking ROS generation, inhibiting changes in MMP, and inhibiting the caspase, ATM, p38 and JNK pathways. |
| Databáze: | OpenAIRE |
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