The effects of metabolic inhibitors on the contraction of creatine-depleted muscle
| Autor: | B. Eiselt, G. W. Mainwood, M. Alward |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
medicine.medical_specialty Contraction (grammar) Physiology Antimetabolites Stimulation Biology Creatine Guanidines chemistry.chemical_compound Adenosine Triphosphate Physiology (medical) Internal medicine Sodium Cyanide Respiration Pi medicine Animals Glycolysis Creatine Kinase Pharmacology Tissue Extracts Rats Inbred Strains General Medicine Phosphate Rats Endocrinology chemistry Phosphorylation Propionates Muscle Contraction |
| Zdroj: | Canadian journal of physiology and pharmacology. 60(2) |
| ISSN: | 0008-4212 |
| Popis: | Rats were fed on a diet containing 1% β-guanidinopropionate (Gp) to deplete their muscles of creatine. The apparent energy reserves (creatine phosphate (CrP) + ATP) of rested state diaphragm muscle strips were found to be 79% depleted by this treatment. To determine if the effective energy reserves for contraction were depleted to a similar extent, the response to direct electrical stimulation (0.2-s tetani) was measured in the presence of inhibitors of respiration (NaCN) and glycolysis (iodoacetate). Only 4 ± 1 contractions could be elicited from strips from Gp-fed animals. Normal strips gave 15 ± 2 contractions under the same conditions. For both sets of diaphragms the energetic cost of contraction in terms of ~P was approximately 1 μmol/g wet weight. The mean level of Pi generated following stimulation to exhaustion was 10.1 μmol/g more in normal than in depleted strips. It is concluded that no significant additional energy stores such as phosphorylated Gp are readily available for contraction in muscles depleted of creatine by Gp treatment. |
| Databáze: | OpenAIRE |
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