Effect of human milk on type 1 and P-fimbrial mRNA expression in intestinal Escherichia coli strains
| Autor: | H-J Monstein, Forough L. Nowrouzian, Agnes E. Wold, Ingegerd Adlerberth |
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| Rok vydání: | 2004 |
| Předmět: |
Operon
Fimbria Virulence medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Escherichia Gene expression medicine Escherichia coli Humans RNA Messenger biology Integrases Milk Human Reverse Transcriptase Polymerase Chain Reaction Escherichia coli Proteins Infant Newborn Infant Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology Enterobacteriaceae Bacterial adhesin DNA-Binding Proteins Intestines Genes Bacterial Child Preschool Fimbriae Bacterial bacteria Genes Switch |
| Zdroj: | Letters in applied microbiology. 40(1) |
| ISSN: | 0266-8254 |
| Popis: | Aims: Escherichia coli from breastfed infants express more type 1 fimbriae and less P fimbriae than E. coli from bottle-fed infants. In this study we investigated the effect of human milk on production of mRNA for fimA (type 1 fimbriae) and papC (P fimbriae) in E. coli. Methods and Results: Production of adhesin gene mRNA was estimated using a reverse transcriptase polymerase chain reaction in E. coli strains under different culture conditions. More type 1 fimbrial mRNA was produced after culture in human milk (P = 0.001) or Luria broth (P = 0.014) than after culture on agar, whereas P-fimbrial mRNA production was similar under all tested growth conditions. When cultured on agar, E. coli strains carrying both the fim and pap operons produced less type 1 and P-fimbrial mRNA than strains that had only the fim or pap operons, respectively (P = 0.03 and 0.056). Significance and Impact of the Study: Environmental regulation of adhesin expression may be influenced by cross-talk between fimbrial operons. |
| Databáze: | OpenAIRE |
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