Identification and characterization of the CRPV E7 protein expressed in COS-7 cells
| Autor: | Miguel S. Barbosa, Felix O. Wettstein |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Immunoprecipitation
Viral protein Recombinant Fusion Proteins viruses Heterologous Kidney medicine.disease_cause Cottontail rabbit papillomavirus Cell Line Retinoblastoma-like protein 1 Viral Proteins Virology Chlorocebus aethiops Protein A/G medicine Animals Papillomaviridae Antiserum biology Fibroblasts Cell Transformation Viral Molecular biology Fusion protein biology.protein Protein G Subcellular Fractions |
| Zdroj: | Virology. 165:134-140 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(88)90666-6 |
| Popis: | The papillomavirus E7 protein may play an important role in oncogenesis as it is the major viral protein present in human cervical cancer-derived cell lines. Because of the relevance of the cottontail rabbit papillomavirus (CRPV) system for the study of viral-induced malignancies, we characterized its E7 protein expressed by a heterologous strong promoter. An E7-specific antiserum was obtained by immunizing rabbits with a Trp E-E7 fusion protein containing the 88 carboxyl-terminal amino acids of E7. This antiserum specifically precipitated from [ 35 ]S]cysteine but not from 32 PO 4 -labeled transiently transfected COS-7 cells a 14-kDa protein. The protein was present only in the soluble cytoplasmic fraction and exhibited a heterogeneous sedimentation rate in nondenaturing glycerol gradients. |
| Databáze: | OpenAIRE |
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