A rapid colorimetric assay of killer toxin activity in yeast
| Autor: | Valerie J. Hodgson, Graeme M. Walker, David Button |
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| Rok vydání: | 1994 |
| Předmět: |
Time Factors
Tetrazolium Salts Microbial Sensitivity Tests medicine.disease_cause Microbiology Colorimetry (chemical method) Redox indicator chemistry.chemical_compound Genetics medicine MTT assay Molecular Biology Candida Formazans Candida glabrata biology Toxin Biological activity Mycotoxins biology.organism_classification Killer Factors Yeast Yeast Thiazoles chemistry Biochemistry Colorimetry sense organs Formazan Oxidation-Reduction |
| Zdroj: | FEMS Microbiology Letters. 120:201-205 |
| ISSN: | 1574-6968 0378-1097 |
| Popis: | The pale yellow redox indicator 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is reduced to a dark blue end-product, MTT-Formazan, by the mitochondrial dehydrogenases of living cells. MTT reduction can be measured spectrophotometrically at a wavelength of 570 nm and a method is described to assay the cidal activity of Williopsis mrakii killer toxin against sensitive cells of Candida glabrata. The MTT assay is rapid, quantitative and compares favourably with traditional plating techniques for the assessment of sensitive viability. |
| Databáze: | OpenAIRE |
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