Spatiotemporal heterogeneity of tumor vasculature during tumor growth and antiangiogenic treatment: MRI assessment using permeability and blood volume parameters

Autor:	Woo Hyun Shim, Jeong Kon Kim, Sang-Yeob Kim, Changhoe Heo, Gyunggoo Cho, Chang Kyung Lee, Bum Woo Park, Dong-Cheol Woo, Cherry Kim, Yun Jae Kim, Do-Wan Lee, Dong-Jun Bae, Ji-Yeon Suh
Rok vydání:	2018
Předmět:	Male 0301 basic medicine CD31 Cancer Research Pathology medicine.medical_specialty medicine.drug_class Angiogenesis Inhibitors Antineoplastic Agents Blood volume tumor vessels Tyrosine-kinase inhibitor Capillary Permeability Mice 03 medical and health sciences 0302 clinical medicine Text mining Neoplasms medicine Extracellular Animals Humans Radiology Nuclear Medicine and imaging Original Research Cancer Biology Blood Volume Neovascularization Pathologic medicine.diagnostic_test business.industry spatial heterogeneity Magnetic resonance imaging Immunohistochemistry Magnetic Resonance Imaging Xenograft Model Antitumor Assays Phenotype Tumor Burden Disease Models Animal 030104 developmental biology Oncology Permeability (electromagnetism) 030220 oncology & carcinogenesis business Biomarkers
Zdroj:	Cancer Medicine
ISSN:	2045-7634
DOI:	10.1002/cam4.1624
Popis:	Tumor heterogeneity is an important concept when assessing intratumoral variety in vascular phenotypes and responses to antiangiogenic treatment. This study explored spatiotemporal heterogeneity of vascular alterations in C6 glioma mice during tumor growth and antiangiogenic treatment on serial MR examinations (days 0, 4, and 7 from initiation of vehicle or multireceptor tyrosine kinase inhibitor administration). Transvascular permeability (TP) was quantified on dynamic‐contrast‐enhanced MRI (DCE‐MRI) using extravascular extracellular agent (Gd‐DOTA); blood volume (BV) was estimated using intravascular T2 agent (SPION). With regard to region‐dependent variability in vascular phenotypes, the control group demonstrated higher TP in the tumor center than in the periphery, and greater BV in the tumor periphery than in the center. This distribution pattern became more apparent with tumor growth. Antiangiogenic treatment effect was regionally heterogeneous: in the tumor center, treatment significantly suppressed the increase in TP and decrease in BV (ie, typical temporal change in the control group); in the tumor periphery, treatment‐induced vascular alterations were insignificant and BV remained high. On histopathological examination, the control group showed greater CD31, VEGFR2, Ki67, and NG2 expression in the tumor periphery than in the center. After treatment, CD31 and Ki67 expression was significantly suppressed only in the tumor center, whereas VEGFR2 and α‐caspase 3 expression was decreased and NG2 expression was increased in the entire tumor. These results demonstrate that MRI can reliably depict spatial heterogeneity in tumor vascular phenotypes and antiangiogenic treatment effects. Preserved angiogenic activity (high BV on MRI and high CD31) and proliferation (high Ki67) in the tumor periphery after treatment may provide insights into the mechanism of tumor resistance to antiangiogenic treatment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df05c1c13f26a334140383f61e1af2e9 https://doi.org/10.1002/cam4.1624 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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