Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting
| Autor: | Dopico, Eva, Del-Rei, Rodrigo Pimenta, Espinoza, Bertha, Ubillos, Itziar, Zanchin, Nilson Ivo Tonin, Sulleiro Igual, Elena, Moure García, Zaira, Celedon, Paola Alejandra Fiorani, Souza, Wayner Vieira, da Silva, Edimilson Domingos, Gomes, Yara Miranda, Santos, Fred Luciano Neves, Universitat Autònoma de Barcelona |
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| Přispěvatelé: | Institut Català de la Salut, [Dopico E, Ubillos I] Laboratori Clínic Territorial Metropolitana Sud, Institut Català de la Salut, Barcelona, Spain. [Del-Rei RP] Faculty of Technology and Sciences of Bahia, Salvador, Bahia, Brazil. [Espinoza B] Instituto de Investigaciones Biomédicas, Departamento de Inmunología, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. [Zanchin NIT] Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Paraná, Brazil. [Sulleiro E, Moure Z] Servei de Microbiologia, Hospital Universitari Vall d'Hebron, Barcelona, Spain., Vall d'Hebron Barcelona Hospital Campus |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Chimeric antigens Chagas disease Antibodies Protozoan Chagas Malaltia de - Barcelona Serology enfermedades parasitarias::infecciones por protozoos::infecciones por Euglenozoa::tripanosomiasis::enfermedad de Chagas [ENFERMEDADES] Other subheadings::/statistics & numerical data [Other subheadings] 0302 clinical medicine Immigrants - Malalties - Diagnòstic Pregnancy Medicine Persons::Emigrants and Immigrants [NAMED GROUPS] Otros calificadores::/estadística & datos numéricos [Otros calificadores] 030212 general & internal medicine personas::emigrantes e inmigrantes [DENOMINACIONES DE GRUPOS] Accuracy Immunoassay biology Antígens - Immunologia Infectious Diseases Female Antibody Toxoplasma Parasitic Diseases::Protozoan Infections::Euglenozoa Infections::Trypanosomiasis::Chagas Disease [DISEASES] Research Article Barcelona Adult Trypanosoma cruzi 030106 microbiology Emigrants and Immigrants Antigens Protozoan Enzyme-Linked Immunosorbent Assay Cross Reactions Sensitivity and Specificity lcsh:Infectious and parasitic diseases 03 medical and health sciences Antigen parasitic diseases Humans lcsh:RC109-216 Biological Factors::Antigens::Antigens Protozoan [CHEMICALS AND DRUGS] business.industry Toxoplasma gondii biology.organism_classification medicine.disease Parasitology Spain Immunology biology.protein business Trypanosomiasis factores biológicos::antígenos::antígenos protozoarios [COMPUESTOS QUÍMICOS Y DROGAS] |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona BMC Infectious Diseases, Vol 19, Iss 1, Pp 1-7 (2019) BMC Infectious Diseases Scientia |
| Popis: | Chagas disease; Chimeric antigens; Trypanosoma cruzi Malaltia de Chagas; Antígens quimèrics; Trypanosoma cruzi Enfermedad de Chagas; Antígenos quiméricos; Trypanosoma cruzi BACKGROUND: Chronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8.1 and IBMP-8.4 chimeric antigens (Molecular Biology Institute of Paraná - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi-infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease. METHODS: Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house automated ELISA with 347 positive and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from pregnant women with Toxoplasma gondii (n = 98) and Zika virus (n = 75) antibodies. RESULTS: The area under the curve values was 1 and 0.99 for the IBMP-8.1 and IBMP-8.4 proteins, respectively, demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8.1 than IBMP-8.4 in positive samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from 99.4% for IBMP-8.1 to 99.1% for IBMP-8.4 and was not statistically different. Specificity for IBMP-8.1 reached 100 and 99.7% for IBMP-8.4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was similar to that for negative Chagas disease individuals. CONCLUSIONS: Our results showed an outstanding performance of IBMP-8.1 and IBMP-8.4 chimeric antigens by ELISA and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in non-endemic settings. |
| Databáze: | OpenAIRE |
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