Influence of physiological concentrations of androgens on the developmental competence and sex ratio of in vitro produced bovine embryos

Autor: C. K. Hamilton, Pawel M. Bartlewski, Angus D. Macaulay, W. Allan King
Rok vydání: 2013
Předmět:
Zdroj: Reproductive Biology. 13:41-50
ISSN: 1642-431X
DOI: 10.1016/j.repbio.2013.01.175
Popis: This study was designed to determine if the addition of androgens at ovarian follicular fluid (FF) concentrations to oocyte maturation media would alter the development and sex ratio of bovine embryos. To maximize hormone bioavailability, oil was removed and glass culture dishes were used during in vitro maturation (IVM) phase; this modified system was then used in the present experiment along with the standard IVM system utilizing plastic containers and incubation under oil. Ethanol (0.2%) was the vector for steroid hormone delivery. Oocytes were incubated for 22h in the presence of two doses ("low" and "high") of androstenedione (A4) or testosterone (T); the doses were based on the concentrations of both androgens in preovulatory bovine follicles (A4: 337.5 and 562.5ng/ml; T: 22.2 and 42.6ng/ml). The results of hormone assays indicated that bioavailability of steroid hormones remained relatively constant, regardless of the IVM system used. The plasticware with the addition of T resulted in significantly higher cleavage rates (80.0±2.1%) than any other combination of treatments (plasticware×A4: 71.5±2.6%; glassware×T: 71.2±1.9%; and glassware×A4: 71.4±2.4%). The blastocyst formation rate for the plasticware×T treatment (39.7±2.5%) was significantly greater than for all other combinations (glassware×T: 28.7±2.2%; glassware×A4: 24.0±2.8%; and plasticware×A4: 19.8±3.0%) and the low dose of T (37.1±2.5%) resulted in higher (p
Databáze: OpenAIRE
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