Retinoic acid effect on cyclic AMP-dependent protein kinases in embryonal carcinoma cells: studies with differentiation-defective sublines
| Autor: | Ariane Plet, Wayne B. Anderson, Danièle Evain Brion, Pascale Gerbaud, Michael I. Sherman |
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| Rok vydání: | 1986 |
| Předmět: |
Embryonal Carcinoma Stem Cells
Physiology Clinical Biochemistry Retinoic acid Retinoic acid receptor beta Cell Differentiation Tretinoin Cell Biology Retinoic acid receptor gamma Biology Retinoic acid-inducible orphan G protein-coupled receptor Cell biology Biomechanical Phenomena Cell Line Retinoic acid receptor chemistry.chemical_compound Mice Biochemistry chemistry Retinoic acid receptor alpha Neoplastic Stem Cells CAMP binding Animals Protein kinase A Protein Kinases |
| Zdroj: | Journal of cellular physiology. 127(2) |
| ISSN: | 0021-9541 |
| Popis: | Retinoic acid induces the differentiation of PCC4.aza 1R and Nulli-SCC1 embryonal carcinoma (EC) cells. In response to retinoic acid treatment, the levels of cyclic AMP (cAMP)-dependent protein kinases are enhanced in the plasma membrane within 17 hours and in the cytosol fractions of these cells within 2 to 3 days, as determined by phosphotransferase activity and by 8-azido-cyclic [32P]AMP binding to the RI and RII regulatory subunits. PCC4 (RA)-1 and Nulli (RA)-1 are mutant EC lines that fail to differentiate in response to retinoic acid. The former line, but not the latter, lacks cellular retinoic acid-binding protein (cRABP). Basal levels of cAMP-dependent protein kinase activities are elevated in PCC4 (RA)-1 cells. When these cells are treated with retinoic acid, neither cAMP-dependent protein kinase activities nor cAMP binding activities are enhanced; rather, there is a decrease in cytosolic kinase activity and RI subunit. On the other hand, Nulli (RA)-1 cells exhibit increases both in cAMP-dependent protein kinase activities and cAMP binding in response to retinoic acid. These results raise the possibility that cRABP mediates the enhancement of regulatory and catalytic subunits of cAMP-dependent protein kinases in both the membrane and the cytosolic fractions of the teratocarcinoma cells. There also might be some effects of retinoic acid on the cAMP-dependent protein kinase that are unrelated to differentiation and to the presence of cRABP. |
| Databáze: | OpenAIRE |
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