| Popis: |
Objective: To investigate the effects of sotetsuflavone on endostatin, transforming growth factor-β (TGF-β), signal transducers and activators of transcription 3 (STAT3), β-catenin and zonula occludens-1 protein (ZO-1) in non-small cell lung cancer A549 cells. Methods: STAT3, β-catenin, TGF-β and ZO-1 mRNA expression were detected by real-time PCR. Endostatin and TGF-β expression were detected by immunofluorescence assay. STAT3 and β-catenin protein expression were detected by western blot. Results: Compared with the control group, TGF-β, STAT3 and β-catenin expression were down-regulated, endostatin and ZO-1 expression were up-regulated by sotetsuflavone. Simultaneously, it showed a significant concentration-dependent. Conclusion: The mechanism of action of sotetsuflavone in the treatment of lung cancer may be via inhibiting the expression of TGF-β, STAT3, and β-catenin, increasing the expression of endostatin and ZO-1, thereby exerting an anti-tumor effect. |
