Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought Stress
| Autor: | Sultana eRasheed, Khurram eBashir, Akihiro eMatsui, Maho eTanaka, Motoaki eSeki |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis thaliana Drought tolerance Plant Science drought Biology lcsh:Plant culture 01 natural sciences Transcriptome abscisic acid 03 medical and health sciences chemistry.chemical_compound Arabidopsis transcription factors Botany lcsh:SB1-1110 Gene Abscisic acid Cell wall modification Original Research fungi food and beverages biology.organism_classification abiotic stresses 030104 developmental biology chemistry Shoot microarray 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Vol 7 (2016) |
| ISSN: | 1664-462X |
| Popis: | Drought stress has a negative impact on crop yield. Thus, understanding the molecular mechanisms responsible for plant drought stress tolerance is essential for improving this beneficial trait in crops. In the current study, a transcriptional analysis was conducted of gene regulatory networks in roots of soil-grown Arabidopsis plants in response to a drought stress treatment. A microarray analysis of drought-stressed roots and shoots was performed at 0, 1, 3, 5, 7 and 9 days. Results indicated that the expression of many drought stress-responsive genes and abscisic acid biosynthesis-related genes was differentially regulated in roots and shoots from days 3 to 9. The expression of cellular and metabolic process-related genes was up-regulated at an earlier time-point in roots than in shoots. In this regard, the expression of genes involved in oxidative signaling, chromatin structure, and cell wall modification also increased significantly in roots compared to shoots. Moreover, the increased expression of genes involved in the transport of amino acids and other solutes; including malate, iron, and sulfur, was observed in roots during the early time points following the initiation of the drought stress. These data suggest that plants may utilize these signaling channels and metabolic adjustments as adaptive responses in the early stages of a drought stress. Collectively, the results of the present study increases our understanding of the differences pertaining to the molecular mechanisms occurring in roots versus shoots in response to a drought stress. Furthermore, these findings also aid in the selection of novel genes and promoters that can be used to potentially produce crop plants with increased drought tolerance. |
| Databáze: | OpenAIRE |
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