Regeneration of Functional Adrenal Tissue Following Bilateral Adrenalectomy
| Autor: | L. Sorski, Lee Shaashua, Eli Golomb, Pini Matzner, Ella Rosenne, Neta Gotlieb, Shamgar Ben-Eliyahu, Ely Albaz, Amit Benbenishty, Benjamin Amram |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Restraint Physical 0301 basic medicine Aging medicine.medical_specialty Medullary cavity Hormone Replacement Therapy Injections Subcutaneous Adipose tissue 030209 endocrinology & metabolism Random Allocation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Stress Physiological Corticosterone Internal medicine Adrenal Glands medicine Animals Regeneration Chronic stress Secretion Postoperative Period Research Articles business.industry Regeneration (biology) Margins of Excision Adrenalectomy Survival Analysis Rats Inbred F344 030104 developmental biology chemistry Rats Inbred Lew Adrenal Cortex Female Bilateral adrenalectomy business hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Endocrinology. 159:248-259 |
| ISSN: | 1945-7170 |
| DOI: | 10.1210/en.2017-00505 |
| Popis: | It is assumed that after complete bilateral adrenalectomy (ADX), no adrenal tissue will redevelop and adrenal hormone levels will remain low and unaffected by stress. However, anecdotal observations in animals and in patients suggest that under some unknown circumstances the opposite can occur. Herein, we studied whether adrenalectomized rats can develop an alternative source of systemic corticosterone after complete bilateral ADX with minimal replacement therapy. Male and female rats underwent either a standard ADX, in which the glands were removed with minimal surrounding adipose tissue, or an extensive ADX, in which glands were removed with most surrounding adipose tissue. Excised glands were histologically tested for completeness, and corticosterone replacement was nullified within 1 to 3 weeks postoperatively. In four experiments and in both excision approaches, some rats gradually reestablished baseline corticosterone levels and stress response in a time-dependent manner, but differences were observed in the reestablishing rates: 80% in standard ADX vs 20% in extensive ADX. Upon searching for the source of corticosterone secretion, we were surprised to find functional macroscopic foci of adrenocortical tissue without medullary tissue, mostly proximal to the original location. Chronic stress accelerated corticosterone level reestablishment. We hypothesized that underlying this phenomenon were preexisting ectopic microscopic foci of adrenocortical-like tissue or a few adrenal cells that were pre-embedded in surrounding tissue or detached from the excised gland upon removal. We concluded that adrenalectomized animals may develop compensatory mechanisms and suggest that studies employing ADX consider additional corticosterone supplementation, minimize stress, and verify the absence of circulating corticosterone. |
| Databáze: | OpenAIRE |
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