Xenogeneic antisera against T-lymphocyte receptors recognizing allogeneic transplantation antigens: preparation and properties
| Autor: | N.A. Kraskina, Sh. Gruner, E.I. Rubakova, V.G. Nesterenko |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
C57BL/6
Male Adoptive cell transfer T-Lymphocytes Immunology Receptors Antigen T-Cell chemical and pharmacologic phenomena Thymus Gland Graft vs Host Reaction Mice H-2 Antigens Immune system Antigen Isoantibodies Animals Antibody-Producing Cells Antiserum Mice Inbred BALB C biology Immune Sera T lymphocyte biology.organism_classification Cytotoxicity Tests Immunologic Molecular biology Mice Inbred C57BL Phenotype biology.protein Mice Inbred CBA Female Lymph Nodes Rabbits Antibody Spleen |
| Zdroj: | Cellular immunology. 69(2) |
| ISSN: | 0008-8749 |
| Popis: | Antireceptor sera ARS-1 (anti-CBA-anti-C57BL/6) and ARS-2 (anti-C57BL/6-anti-BALB/c) were prepared in a xenogeneic system by immunizing rabbits with alloimmune CBA anti-C57BL/6 and C57BL/6 anti-BALB/c lymphocytes and subsequent exhaustive absorption of rabbit immune sera with red blood cells, lymphocytes, liver cells, and serum from intact mice. In the cytotoxicity test, both ARS sera lysed only activated T lymphocytes of corresponding, but no other specificities, and did not affect intact lymphocytes. They did not inactivate in vitro the PFC-producing antibodies to SRBC or inhibit the immune response to SRBC and Viantigen of Salmonella typhi in adoptive transfer experiments. Pretreatment of intact CBA lymphocytes in vitro with ARS-1 plus complement suppressed their ability to induce lethal GvH disease in irradiated (CBA × C57BL/6)F1, but not (CBA × BALB/c)F1 recipients; similar pretreatment of C57BL/6 lymphocytes did not lower lethality among irradiated (CBA × C57BL/6)F1 recipients. This method of preparing ARS may help to obtain highly specific antireceptor sera for regulation of the immune response to different antigens in outbred animals and in humans. |
| Databáze: | OpenAIRE |
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