On-chip absorption measurements using an integrated waveguide
| Autor: | Bryan G. Splawn, Fred E. Lytle |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Boron Compounds
Silicon Sensitivity and Specificity Biochemistry Fluorescence Absorption Analytical Chemistry law.invention Optics law Electrochemistry System on a chip Dimethylpolysiloxanes Absorption (electromagnetic radiation) Total internal reflection Miniaturization business.industry Chemistry Detector Electrophoresis Capillary Reproducibility of Results Equipment Design Chip Refractometry Calibration Microscopy Electron Scanning Reflection (physics) Fluorescein business Waveguide Refractive index |
| Zdroj: | Analytical and Bioanalytical Chemistry. 373:519-525 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-002-1316-9 |
| Popis: | Square hollow waveguides are used to integrate measurement of absorption with chip-based electrophoresis. The 50x50 microm liquid channel and 50x50 microm waveguide are etched as a negative pattern into a silicon master and replicated as a positive in poly-dimethylsiloxane (PDMS). The uniform refractive index of the chip prevents guiding by total internal reflection. Instead, light at 488 nm is guided by reflection at the air-PDMS interface. The waveguide has a 60% efficiency over a distance of 3.2 cm. Separation of fluorescein and the dye BODIPY is demonstrated. A detection limit (S/N=3) of 200 microM fluorescein is obtained using a 50 microm pathlength and a simple photocell detector. |
| Databáze: | OpenAIRE |
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