Dietary protein composition influences abundance of peptide and amino acid transporter messenger ribonucleic acid in the small intestine of 2 lines of broiler chicks
| Autor: | Eric A. Wong, Huifeng Li, Derek A. Emmerson, Elizabeth R. Gilbert, Kenneth E. Webb |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Whey protein Amino Acid Transport Systems Duodenum TRPV Cation Channels Peptide Transporter 1 Polymerase Chain Reaction Hydrolysate Intestinal absorption Ileum Intestine Small medicine Animals RNA Messenger Amino acid transporter Amino Acids chemistry.chemical_classification Symporters biology Peptide transporter 1 Transporter General Medicine Animal Feed Molecular biology Small intestine Amino acid Excitatory Amino Acid Transporter 3 Jejunum Milk medicine.anatomical_structure Intestinal Absorption Biochemistry chemistry biology.protein Animal Science and Zoology Dietary Proteins Peptides Chickens |
| Zdroj: | Poultry Science. 89:1663-1676 |
| ISSN: | 0032-5791 |
| DOI: | 10.3382/ps.2010-00801 |
| Popis: | This study evaluated the effect of dietary protein composition on mRNA abundance of a peptide transporter (peptide transporter 1, PepT1), amino acid (AA) transporters [Na(+)-independent cationic and zwitterionic AA transporter (b(o,+)AT), excitatory AA transporter 3 (EAAT3), Na(+)-independent cationic and Na(+)-dependent neutral AA transporter 2 (y(+)LAT2), L-type AA transporter 1 (LAT1), and cationic AA transporter 1 (CAT1)], and a digestive enzyme (aminopeptidase N) in 2 lines (A and B) of broilers that differentially express PepT1 mRNA (line B > line A). From d 8 to 15 posthatch, birds were fed 1 of 3 diets. Protein sources included whey protein concentrate, a whey partial hydrolysate (WPH), or a mixture of free AA (AA) identical to the composition of whey. Quantities of mRNA were assayed by real-time PCR in the small intestine of males at d 8, 9, 11, 13, and 15. For all genes except LAT1, abundance of mRNA was greatest in line B birds that consumed the WPH diet (P < 0.006). When mRNA abundance was normalized to beta-actin quantities, this effect disappeared, demonstrating a generalized effect on gene expression in line B birds that consumed the hydrolysate. There was a greater villus height:crypt depth ratio (P < 0.05) in line B birds fed the WPH diet as compared with line A. In conclusion, line B birds, which express greater PepT1, displayed enhanced intestinal mucosal absorptive surface area and differential regulation of PepT1, AA transporters, and aminopeptidase N in response to dietary protein composition. |
| Databáze: | OpenAIRE |
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