EU Drinking Water Directive reference methods for enumeration of total coliforms and Escherichia coli compared with alternative methods
| Autor: | P.J. Nobel, Abraham Brouwer, F.M. Schets, G.B. Engels, K.A. Mooijman, S. Strating |
|---|---|
| Přispěvatelé: | Chemistry and Biology, Institute for Environmental Studies |
| Rok vydání: | 2002 |
| Předmět: |
Alternative methods
food.ingredient Colony Count Microbial Clean water Biology medicine.disease_cause Applied Microbiology and Biotechnology Culture Media Microbiology Europe food Enterobacteriaceae Predictive Value of Tests Water Supply Direct plating Drinking water directive Escherichia coli Colony count Enumeration medicine Agar Food science |
| Zdroj: | Letters in Applied Microbiology, 34(3), 227-231. Wiley-Blackwell Schets, F M, Nobel, P J, Strating, S, Mooijman, K A, Engels, G B & Brouwer, A 2002, ' EU Drinking Water Directive reference methods for enumeration of total coliforms and Escherichia coli compared with alternative methods ', Letters in Applied Microbiology, vol. 34, no. 3, pp. 227-231 . https://doi.org/10.1046/j.1472-765x.2002.01075.x |
| ISSN: | 0266-8254 |
| DOI: | 10.1046/j.1472-765x.2002.01075.x |
| Popis: | Aims: The reference methods for enumeration of total coliforms and Escherichia coli as stated in the European Drinking Water Directive were compared with alternative methods. Methods and Results: Laboratories used the reference method on Lactose TTC agar (LTTC), the Colilert®/18 system, Laurysulphate Agar (LSA), Chromocult® Coliform Agar and the E. coli Direct Plating (DP) method. They enumerated more total coliforms on LTTC than on LSA. Conclusions: LTTC is suitable for analysis of very clean water samples only, due to heavy background growth. Colilert®/18 is a good alternative but it enumerates a broader group of total coliforms, resulting in higher counts. The DP method appeared to be the best choice for enumeration of E. coli because Colilert®/18 produces lower counts and false-negative results. Significance and Impact of the Study: This study shows the limitations of the EU reference method on LTTC due to lack of selectivity and suggests alternative methods for the enumeration of total coliforms and E. coli. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text