Novel Function of Isoamylamine Improves Survival in Endotoxemic Mice by Ameliorating Coagulopathy and Attenuating MMP-9 Expression Through p-ERK/p-p38 Signaling at Early Stage
| Autor: | Yong-Ren Yen, Lina Wang, Soo-Ray Wang, Yu-Hsun Wang, Fung-Jou Lu, Lien-Cheng Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine 030204 cardiovascular system & hematology Pharmacology Matrix metalloproteinase Critical Care and Intensive Care Medicine p38 Mitogen-Activated Protein Kinases Mice 03 medical and health sciences 0302 clinical medicine hemic and lymphatic diseases medicine Coagulopathy Animals Amines Stage (cooking) Isoamylamine Extracellular Signal-Regulated MAP Kinases Blood Coagulation Disseminated intravascular coagulation Mice Inbred BALB C Septic shock business.industry Blood Coagulation Disorders medicine.disease Shock Septic Endotoxemia 030104 developmental biology Matrix Metalloproteinase 9 Shock (circulatory) Emergency Medicine medicine.symptom business Function (biology) circulatory and respiratory physiology |
| Zdroj: | Shock. 47:772-779 |
| ISSN: | 1073-2322 |
| DOI: | 10.1097/shk.0000000000000786 |
| Popis: | When a host suffers endotoxemic shock or septic shock, it results in many symptoms including disseminated intravascular coagulation (DIC). Septic shock (SS) causes coagulation time to decrease and then gradually increase, finally becoming prolonged and giving rise to DIC. Isoamylamine (IA) is one of the main components of grape products and can improve the survival rate of endotoxin lipopolysaccharide (LPS)-induced endotoxemic shock. The aim of this study was to elucidate if IA ameliorates coagulopathy in the early phase of LPS-induced damage. We studied the effects of IA on the coagulation system of extrinsic (prothrombin time [PT]) and intrinsic (activated partial thromboplastin time [aPTT]) pathways. PT and aPTT were tested in plasma drawn from mice following intraperitoneal (IP) injection of 1 mL of 1,000 ppm IA after LPS administration. Shortened PT was ameliorated by 1,000 ppm IA 1 h after LPS administration, but there was no effect on aPTT. In conclusion, IA 1,000 ppm partially intervenes in the early phase of LPS-induced damage, shortening plasma PT 1 h after LPS treatment in mice. Furthermore, we found 1,000 ppm IA also could attenuate MMP-9 expression through p-ERK/p-p38 signaling in mice hepatocyte extracts. This study focused on the effects of IA on blood coagulation function and inflammatory proteins. In the current situation of absence of effective treatment for SS, IA can increase survival rate and may offer another choice of patient avoiding causing death during endotoxemic shock. |
| Databáze: | OpenAIRE |
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