Comparison of Some Relative Gene Expressions in the Yolk Sac Membrane and Small Intestine of the Embryos in the Layer Hybrid and Pure Breeds
| Autor: | Ömer Faruk Güngör, Metin Erdogan, S. Erol, E. E. Onbaşılar, Mücahit Kahraman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures Embryonic age Biology layer hybrid Andrology 03 medical and health sciences lcsh:Zoology Gene expression medicine lcsh:QL1-991 Yolk sac lcsh:SF1-1100 pure breed lcsh:Veterinary medicine Hatching 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences 040201 dairy & animal science Small intestine 030104 developmental biology medicine.anatomical_structure embryonic structures biology.protein gene expression lcsh:SF600-1100 Animal Science and Zoology lcsh:Animal culture Digestion GLUT5 |
| Zdroj: | Brazilian Journal of Poultry Science v.20 n.3 2018 Brazilian Journal of Poultry Science Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) instacron:FACTA Brazilian Journal of Poultry Science, Vol 20, Iss 3, Pp 435-440 |
| Popis: | Pure breeds are important due to the fact that they adapt well to poor husbandry conditions. However, gene expression of digestive enzymes and nutrient transporters of pure breeds and layer hybrids have not been studied yet. Therefore, this study was directed to detect the gene expression of digestive enzymes and nutrient transporters in the yolk sac membrane and small intestine of the embryos in layer hybrid and pure breeds. A total of 354 hatching eggs were collected from 29 wk old Denizli, Gerze and Lohman White (LW) breeder flocks. Twelve eggs from each genotype were selected at embryonic day 19 (E19) and day 21 (E21). APN, SI, PepT1, EAAT3, CAT1, B0AT, SGLT1 and GLUT5 mRNA expressions were evaluated in the yolk sac membrane (YSM) and small intestine of the embryos from different genotypes. Expressions of APN and EAAT3 in the small intestine of Denizli embryo were upregulated from E19 to E21. B0AT, PepT1 and SGLT1 expressions were upregulated in the small intestine of pure breed and layer hybrid embryos with increasing embryonic age. SI expression in the small intestine of the LW embryos was upregulated from E19 to E21. APN, B0AT and EAAT3 expressions in the YSM of Denizli embryos were dowregulated from E19 to E21. EAAT3 and SGLT1 expressions in the YSM of Gerze embryos were upregulated at E21 and compared to E19. Taken together, our results indicate that genotype and embryonic age influenced the expression of digestive enzymes and nutrient transporter genes in the small intestine and YSM of embryos. |
| Databáze: | OpenAIRE |
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