Oligodendrocyte Progenitor Proliferation and Maturation Is Differentially Regulated by Male and Female Sex Steroid Hormones
| Autor: | David Raban, Robert P. Skoff, Patrizia Casaccia-Bonnefil, Michela Muggironi, Mireya Marin-Husstege |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Selective Estrogen Receptor Modulators Cytoplasm medicine.medical_specialty medicine.drug_class Estrogen receptor Biology S Phase Mice Myelin Developmental Neuroscience Internal medicine medicine Animals Estrogen Receptor beta Testosterone Progenitor cell Gonadal Steroid Hormones Cells Cultured Progesterone Cell Proliferation Oligodendrocyte progenitor proliferation Sex Characteristics Estradiol Stem Cells Brain Cell Differentiation Oligodendrocyte Oligodendroglia Tamoxifen Endocrinology medicine.anatomical_structure Animals Newborn Neurology Estrogen Female Cell Surface Extensions hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Developmental Neuroscience. 26:245-254 |
| ISSN: | 1421-9859 0378-5866 |
| DOI: | 10.1159/000082141 |
| Popis: | Using primary cultures of oligodendrocyte progenitors isolated from male and female neonatal rodent brains, we observed more oligodendrocytes in female-derived compared to male-derived cultures. To determine whether the observed differences were due to a differential effect of sex hormones on proliferation, we treated cultures with increasing doses of 17beta-estradiol, testosterone or progesterone and labeled cells with bromodeoxyuridine to identify cells in S phase. Treatment with 17beta-estradiol, but not progesterone or testosterone, delayed the exit of oligodendrocyte progenitor cells from the cell cycle. In addition, 17beta-estradiol treatment enhanced membrane sheet formation, while progesterone increased cellular branching. Interestingly, the estrogen modulator tamoxifen mimicked the effect of 17beta-estradiol on cell cycle exit, but not on membrane formation. Immunocytochemical localization of estrogen receptors (ERs) showed ERbeta mainly localized to the cytoplasm of oligodendrocytes, suggesting that the effect of 17beta-estradiol on membrane formation could be mediated by interaction with this receptor. We conclude that sex steroids differentially regulate oligodendrocyte progenitor number and myelin formation, possibly contributing to gender-specific differences in repair. |
| Databáze: | OpenAIRE |
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