Three-axis Modification of Coordinates Enables Accurate Stereotactic Targeting in Non-human Primate Brains of Different Sizes
| Autor: | Hanlim Song, Hyungsun Kim, Hyoung-Ihl Kim, Ra Gyung Kim, Ji-Young Park, Jeong Ho Hwang, Goo-Hwa Kang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Stereotactic surgery Body weight law.invention 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Nonhuman primate law Atlas (anatomy) medicine Stereotaxy Cartesian coordinate system Physics Non human primate Skull 030104 developmental biology medicine.anatomical_structure Brain size Original Article Neurology (clinical) 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Experimental Neurobiology |
| ISSN: | 2093-8144 1226-2560 |
| Popis: | The brain grows with age in non-human primates (NHPs). Therefore, atlas-based stereotactic coordinates cannot be used directly to target subcortical structures if the size of the animal's brain differs from that used in the stereotactic atlas. Furthermore, growth is non-uniform across different cortical regions, making it difficult to simply apply a single brain-expansion ratio. We determined the skull reference lines that best reflect changes in brain size along the X, Y, and Z axes and plotted the changes in reference-line length against the changes in body weight. The skull reference lines had a linear relationship with body weight. However, comparison of skull reference lines with body weight confirmed the non-uniform skull growth during postnatal development, with skull growth more prominent in the X and Y axes than the Z axis. Comparing the differences between the atlas-based lengths and those calculated empirically from plot-based linear fits, we created craniometric indices that can be used to modify stereotactic coordinates along all axes. We verified the accuracy of the corrected stereotactic targeting by infusing dye into internal capsule in euthanized and preserved NHP brains. Our axis-specific, craniometric-index-adjusted stereotactic targeting enabled us to correct for targeting errors arising from differences in brain size. Histological verification showed that the method was accurate to within 1 mm. Craniometric index-adjusted targeting is a simple and relatively accurate method that can be used for NHP stereotactic surgery in the general laboratory, without the need for high-resolution imaging. Graphical Abstract |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|