Plasmodium falciparum: purification of the various gametocyte developmental stages from in vitro-cultivated parasites

Autor:	J K Mwangi, F K Mulaa, Samuel K. Martin, M K Wasunna, J. K. Kiaira, MM Kariuki
Rok vydání:	1998
Předmět:	biology Plasmodium falciparum biology.organism_classification In vitro Apicomplexa Infectious Diseases Biochemistry Virology Botany Centrifugation Density Gradient Research studies Gametocyte Animals Protozoa Parasitology Stage iv Percoll
Zdroj:	The American Journal of Tropical Medicine and Hygiene. 59:505-508
ISSN:	1476-1645 0002-9637
Popis:	Cultivated Plasmodium falciparum gametocytes reach maturity in vitro in approximately 14-16 days, during which they pass through five morphologically distinct developmental stages. Purification of the earlier developmental stages has not been previously reported. We have modified the standard discontinuous Percoll gradient method for the separation of stage IV and V gametocytes to obtain enriched preparations of those and the earlier P. falciparum gametocyte stages. In contrast to the stages II, III, and IV, the mature stage V gametocytes from our gradient readily transformed into gametes. Such preparations may be useful in research studies on the mechanisms that underlie gametocytogenesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dee9eb4c243c358c7a8a641175c0a8d4 https://doi.org/10.4269/ajtmh.1998.59.505 Zobrazit plný text záznamu