Bacterial aetiology of chronic otitis media with effusion in children - risk factors
| Autor: | Karolina Kielbik, Izabela Korona-Glowniak, Agata Wisniewska, Anna Malm, Grazyna Niedzielska, Marek Juda |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_treatment lcsh:Surgery medicine.disease_cause Polymerase Chain Reaction law.invention Microbiology Haemophilus influenzae Moraxella catarrhalis 03 medical and health sciences 0302 clinical medicine law Streptococcus pneumoniae Multiplex polymerase chain reaction otorhinolaryngologic diseases Medicine Humans 030212 general & internal medicine Prospective Studies Original Research Article Tympanostomy tube Carnobacteriaceae 030223 otorhinolaryngology Child Polymerase chain reaction biology Bacteria business.industry Otitis Media with Effusion lcsh:RD1-811 biology.organism_classification Otitis Otorhinolaryngology Effusion Risk factors Child Preschool Chronic Disease Surgery Female medicine.symptom Otopathogens business |
| Zdroj: | Journal of Otolaryngology-Head & Neck Surgery Journal of Otolaryngology-Head and Neck Surgery, Vol 49, Iss 1, Pp 1-7 (2020) |
| ISSN: | 1916-0216 |
| Popis: | Background Otitis media with effusion (OME) may occur spontaneously because of poor Eustachian tube function or as an inflammatory response following AOM. Bacterial involvement in OME has been widely reported, with various available methods to identify pathogens from middle ear effusion, including traditional culture methods and polymerase chain reaction (PCR). Objectives The primary goal of this study was to evaluate the bacteriological profile of middle ear effusion in OME. Risk factors of the bacterial OME aetiology were also identified. Methods Middle ear effusions (MEF) from 50 children, aged 2–8 years, diagnosed by ENT and undergoing routine tympanostomy tube placement were collected. MEF samples were streaked on standard microbiological media. Next, DNA was isolated from MEF samples and analysed with multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Alloiococcus otitidis. Results In multiplex PCR assay 37 (74%) of 50 children were positive for at least one of the four microorganisms. In 27.0% positive children multiple bacterial pathogens were identified. A. otitidis was the most frequently identified in positive MEF children (59.5%). By multiplex PCR, H. influenzae, S. pneumoniae and M. catarrhalis were detected in 24, 18 and 8% of OME patients, respectively. There was significant association between bilateral infection and H. influenzae aetiology of OME. Conclusions Overall we found OME predominantly a single otopathogen infection caused mainly by A. otitidis, which is difficult in identification using standard culture method, ahead to S. pneumoniae and H. influenzae. However, one third of MEF samples had multiple bacterial pathogens. |
| Databáze: | OpenAIRE |
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