The Use of Massive Sequencing to Detect Differences between Immature Embryos of MON810 and a Comparable Non-GM Maize Variety
| Autor: | Maria Pla, Carlos M. Vicient, Emilio Centeno, Pere Puigdomènech, Jose Luis La Paz |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (Espanya), Ministerio de Educación y Ciencia (España), Generalitat de Catalunya |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microarrays
Agricultural Biotechnology Gene Expression lcsh:Medicine Plant Science Transcriptome Gene Expression Regulation Plant Gene expression Cluster Analysis Genome Sequencing Transgenes lcsh:Science Blat de moro -- Genètica Genetics Plant Pests Multidisciplinary Genetically Modified Organisms Gene Expression Regulation Developmental High-Throughput Nucleotide Sequencing Agriculture Genomics Plants Genetically Modified Phenotype Enginyeria genètica vegetal Bioassays and Physiological Analysis Seeds DNA microarray Transgenic plant Sequence Analysis Plant genetic engineering Research Article Biotechnology Transgene Biology Research and Analysis Methods Genes Plant Zea mays Deep sequencing Corn -- Genetics Molecular Biology Techniques Sequencing Techniques Transgenic Plants Molecular Biology Gene Plantes transgèniques Gene Expression Profiling lcsh:R fungi Biology and Life Sciences Computational Biology Genetic Variation Plant Pathology Gene expression profiling Plant Biotechnology lcsh:Q Pest Control |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname PLoS ONE, Vol 9, Iss 6, p e100895 (2014) Digital.CSIC. Repositorio Institucional del CSIC PLoS ONE, 2014, vol. 9, núm. 6, p. e100895 Articles publicats (D-EQATA) DUGiDocs – Universitat de Girona Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona PLoS ONE |
| Popis: | The insect resistant maize YieldGard MON810 was studied to assess the extent to which introduction of a transgene may putatively alter the expression of endogenous genes by comparison of various GM lines vs. their non-transgenic counterparts. To assess the extent to which introduction of a transgene may putatively alter the expression of endogenous genes, GM lines of the insect resistant maize YieldGard MON810 were compared with non-transgenic counterparts. For a more in-depth study, high-throughput deep sequencing together with microarrays were used to compare the transcriptomes of immature embryos of the MON810 variety DKC6575, with a cryIA(b) transgene, and its near-isogenic variety Tietar, grown under controlled environmental conditions. This technique also allows characterisation of the transgenic mRNAs produced. 3′UTR-anchored mRNA-seq produced 1,802,571 sequences from DKC6575 and 1,170,973 from Tietar, which mapped to 14,712 and 14,854 unigenes, respectively. Up to 32 reads from the transgenic embryos matched to the synthetic cry1A(b) sequence, similar to medium-abundant mRNAs. Gene expression analysis, using the R-bioconductor packages EdgeR and DEseq, revealed 140 differentially expressed genes mainly involved in carbohydrate metabolism, protein metabolism and chromatin organisation. Comparison of the expression of 30 selected genes in two additional MON810 and near-isogenic variety pairs showed that most of them were differentially expressed in the three pairs of varieties analysed. Analysis of functional annotation and the precise moment of expression of the differentially expressed genes and physiological data obtained suggest a slight but significant delay in seed and plant maturation of MON810 plants. However, these transcriptomic changes were not associated to undesirable changes in the global phenotype or plant behaviour. Moreover, while most expression changes in MON810 immature embryos were maintained in other transgenic varieties, some gene expression was found to be modulated by the genetic background in which the transgene was introduced through conventional breeding programs. This work was funded by the Spanish MEC project ref. AGL2007-65903/AGR, the CONSOLIDER project and the Xarxa de Referència en Biotecnología of the Generalitat de Catalunya. |
| Databáze: | OpenAIRE |
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