A single mutation in the mammalian orthoreovirus S1 gene is responsible for increased interferon sensitivity in a virus mutant selected in Vero cells
| Autor: | Stéphanie Côté, Guy Lemay, Delphine Lanoie, Emmanuelle Degeorges |
|---|---|
| Přispěvatelé: | Université de Montréal. Faculté de médecine. Département de microbiologie, infectiologie et immunologie |
| Rok vydání: | 2018 |
| Předmět: |
viruses
Mutant Orthoreovirus Mammalian Codon Initiator Genome Viral Biology Reovirus medicine.disease_cause Virus Replication Virus Cell Line 03 medical and health sciences Mice Interferon Virology Cricetinae Chlorocebus aethiops medicine Animals Gene Vero Cells 030304 developmental biology 0303 health sciences Mutation 030302 biochemistry & molecular biology Reverse genetics Reverse Genetics Viral replication Vero cell Capsid Proteins Interferons medicine.drug |
| Zdroj: | Virology. 528 |
| ISSN: | 1096-0341 |
| Popis: | In a previous study, a mammalian orthoreovirus mutant was isolated based on its increased ability to infect interferon-defective Vero cells and was referred to as Vero-cells-adapted virus (VeroAV). This virus exhibits reduced ability to resist the antiviral effect of interferon. In the present study, the complete genome sequence of VeroAV was first determined. Reverse genetics was then used to identify a unique mutation on the S1 gene, overlapping the σ1 and σ1 s reading frame, resulting in increased sensitivity to interferon. A virus lacking σ1 s expression consecutive to mutation of its initiation codon was then shown to exhibit a further increase in sensitivity to interferon, supporting the idea that σ1 s is the viral protein responsible. This identification of a new determinant of reovirus sensitivity to interferon gives credentials to the idea that multiple reovirus genes are responsible for the level of interferon induction and susceptibility to the interferon-induced antiviral activities. |
| Databáze: | OpenAIRE |
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