Effect of Histone Deacetylase HDAC3 on Cytokines IL-18, IL-12 and TNF-α in Patients with Intrahepatic Cholestasis of Pregnancy
| Autor: | Cai-Ru Liu, Yong Shao, Jiao Zheng, Jing Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Physiology
Placenta Severity of Illness Index lcsh:Physiology Pathogenesis 0302 clinical medicine Liver Function Tests Pregnancy Intrahepatic cholestasis of pregnancy lcsh:QD415-436 030219 obstetrics & reproductive medicine integumentary system lcsh:QP1-981 Liver cell musculoskeletal neural and ocular physiology Interleukin-18 Alanine Transaminase Interleukin-12 humanities medicine.anatomical_structure IL-12 030220 oncology & carcinogenesis Immunohistochemistry Female Interleukin 18 Cholestasis of pregnancy IL-18 Signal Transduction Adult medicine.medical_specialty Histone deacetylase 3 Cholestasis Intrahepatic Histone Deacetylases Proinflammatory cytokine Bile Acids and Salts lcsh:Biochemistry 03 medical and health sciences Internal medicine medicine Humans Aspartate Aminotransferases RNA Messenger Tumor Necrosis Factor-alpha business.industry Trophoblast medicine.disease nervous system diseases Pregnancy Complications Endocrinology Gene Expression Regulation Case-Control Studies TNF-α business |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 42, Iss 4, Pp 1294-1302 (2017) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/aims: The pathogenesis of intrahepatic cholestasis of pregnancy (ICP) is poorly understood. Objective: This study aimed to explore the possible effect of HDAC3 (histone deacetylase) on cytokines IL-18, IL-12 and TNF-α in ICP. Methods: Serum levels of cytokines IL-18, IL-12 and TNF-α, bile acids and hepatic function parameters were measured. The expression of HDAC3 in the placenta was determined by immunohistochemistry (IHC), western blotting and RT-PCR. Results: IL-18, IL-12 and TNF-α serum levels were significantly higher in the severe ICP group than in the mild ICP group and the control group, and the difference between the mild ICP group and control group was not significant. HDAC3 protein expression was identified in the nucleus of the placental trophoblast by IHC. HDAC3 mRNA and protein expression were significantly lower in the ICP groups (mild ICP and severe ICP groups) than in the control groups, and no significant difference was found between the mild ICP and severe ICP groups. Conclusions: The low expression of HDAC3 and overexpession of inflammatory cytokines (IL-18, IL-12 and TNF-α) in ICP may be involved in liver cell apoptosis. We suspect that HDAC3 may play an important role in the pathophysiology of ICP. |
| Databáze: | OpenAIRE |
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