Morphology, phylogeny, and sexual stage ofFusarium caatingaenseandFusarium pernambucanum, new species of theFusarium incarnatum-equisetispecies complex associated with insects in Brazil
| Autor: | Neiva Tinti de Oliveira, Antonio Félix da Costa, José Vinícius Correia Trindade, Renan do Nascimento Barbosa, Ana Carla da Silva Santos, Cristiano Souza Lima, Patricia Vieira Tiago |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Fusarium Species complex Physiology 010603 evolutionary biology 01 natural sciences Aleurocanthus woglumi 030308 mycology & parasitology Hemiptera 03 medical and health sciences Phylogenetics Botany Genetics Animals Cluster Analysis Heterothallic Clade Molecular Biology Phylogeny Ecology Evolution Behavior and Systematics 0303 health sciences Genetic diversity Phylogenetic tree biology Cell Biology General Medicine Spores Fungal Genes Mating Type Fungal biology.organism_classification Brazil Multilocus Sequence Typing |
| Zdroj: | Mycologia. 111:244-259 |
| ISSN: | 1557-2536 0027-5514 |
| Popis: | Based on morphological and molecular phylogenetic markers and the fertility of sexual crosses, two novel species of Fusarium associated with Dactylopius opuntiae (Hemiptera: Dactylopiidae) and Aleurocanthus woglumi (Hemiptera: Aleyrodidae) from northeastern Brazil are described as Fusarium caatingaense and F. pernambucanum. Partial sequences of five loci were generated for 29 entomopathogenic Fusarium isolates. Multilocus phylogenetic analyses demonstrated that F. caatingaense and F. pernambucanum belong to the Incarnatum clade of the Fusarium incarnatum-equiseti species complex (FIESC). These species displayed common morphological characters such as the production of various types of aerial conidia formed on monophialides and polyphialides and differ from each other mainly in the dimensions and morphology of their sporodochial conidia. Mating type polymerase chain reaction (PCR) revealed 17 MAT1-1 isolates and 12 MAT1-2 isolates, all of them heterothallic. Fertile perithecia were produced in 4.2% of infraspecific crosses of F. caatingaense and in 13.3% of infraspecific crosses of F. pernambucanum after 2-3 wk. Crosses between F. caatingaense and F. pernambucanum did not result in fertile perithecia. We demonstrate the existence of a sexual stage in species of the Incarnatum clade and describe the morphological characters of these sexual morphs for the first time. These results suggest that previously unknown sexual cycles contribute to the high genetic diversity within FIESC. |
| Databáze: | OpenAIRE |
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