Differential expression and activity of P-glycoprotein and multidrug resistance-associated protein in CD34-positive KG1a leukemic cells
| Autor: | Olivier Fardel, Bernard Drenou, Arnaud Courtois, Renée Fauchet, Léa Payen, B Rault |
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| Rok vydání: | 1998 |
| Předmět: |
Cancer Research
Vincristine Myeloid Molecular Sequence Data Drug Resistance Antigens CD34 HL-60 Cells Drug resistance hemic and lymphatic diseases Activated-Leukocyte Cell Adhesion Molecule medicine Tumor Cells Cultured Humans ATP Binding Cassette Transporter Subfamily B Member 1 P-glycoprotein biology Reverse Transcriptase Polymerase Chain Reaction Daunorubicin medicine.disease Multiple drug resistance Leukemia medicine.anatomical_structure Oncology Leukemia Myeloid Cancer research biology.protein ATP-Binding Cassette Transporters Drug Screening Assays Antitumor Multidrug Resistance-Associated Proteins K562 Cells A431 cells Cell Division medicine.drug |
| Zdroj: | International journal of oncology. 12(2) |
| ISSN: | 1019-6439 |
| Popis: | CD34+ acute myeloid leukemias generally respond poorly to chemotherapy when compared to CD34- myeloid leukemias. In order to contribute to the analysis of the mechanisms involved in this drug resistance, expression and activity of P-glycoprotein (P-gp) and multidrug resistance-associated protein (MRP), two drug efflux pumps conferring multidrug resistance, have been investigated in the CD34+ KG1a leukemic myeloid cell line and in two CD34- K562 and HL60 leukemic myeloid cell lines. Reverse transcription-polymerase chain reaction and dye efflux assays revealed that KG1a cells express P-gp but not MRP whereas neither P-gp nor MRP were detected in K562 and HL60 cells. In addition, KG1a cells were demonstrated to display resistance to anticancer drug substrates for P-gp such as vincristine and daunorubicin and to poorly accumulate vincristine. These results indicated that P-gp, in contrast to MRP, is expressed and functional in the drug-resistant CD34+ KG1a cell line, that may constitute a useful cellular model to analyze the constitutive chemoresistance of CD34+ acute myeloid leukemias. |
| Databáze: | OpenAIRE |
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