Elucidating the Distribution of Plant Metabolites from Native Tissues with Laser Desorption Low-Temperature Plasma Mass Spectrometry Imaging
| Autor: | Cesaré Ovando-Vázquez, Abigail Moreno-Pedraza, Fredd Vergara, Nancy Shyrley Garcia-Rojas, Ignacio Rosas-Román, Nayelli Marsch-Martínez, Jorge Molina-Torres, Robert Winkler, David Díaz-Ramírez, Héctor Guillén-Alonso, Jessica Cuevas-Contreras |
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| Rok vydání: | 2019 |
| Předmět: |
Cactaceae
Nicotine Datura stramonium Nicotiana tabacum medicine.disease_cause Mass spectrometry imaging Analytical Chemistry law.invention chemistry.chemical_compound Alkaloids law Desorption Tobacco medicine Mescaline Chromatography biology Chemistry Tropane Equipment Design Plasma biology.organism_classification Laser Cold Temperature Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Seeds Ultraviolet |
| Zdroj: | Analytical Chemistry. 91:2734-2743 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Secondary metabolites of plants have important biological functions, which often depend on their localization in tissues. Ideally, a fresh untreated material should be directly analyzed to obtain a realistic view of the true sample chemistry. Therefore, there is a large interest for ambient mass-spectrometry-based imaging (MSI) methods. Our aim was to simplify this technology and to find an optimal combination of desorption/ionization principles for a fast ambient MSI of macroscopic plant samples. We coupled a 405 nm continuous wave (CW) ultraviolet (UV) diode laser to a three-dimensionally (3D) printed low-temperature plasma (LTP) probe. By moving the sample with a RepRap-based sampling stage, we could perform imaging of samples up to 16 × 16 cm2. We demonstrate the system performance by mapping mescaline in a San Pedro cactus (Echinopsis pachanoi) cross section, tropane alkaloids in jimsonweed (Datura stramonium) fruits and seeds, and nicotine in tobacco (Nicotiana tabacum) seedlings. In all cases, the ... |
| Databáze: | OpenAIRE |
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