Forced KLF4 expression increases the generation of mature plasma cells and uncovers a network linked with plasma cell stage
| Autor: | Anja Seckinger, Alboukadel Kassambara, Matthieu Schoenhals, Jérôme Moreaux, Véronique Pantesco, Bernard Klein, Michel Jourdan, Dirk Hose |
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| Přispěvatelé: | Department of Biological Hematology , CHU Montpellier , Montpellier, Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Medizinische Klinik und Poliklinik V, Universitätsklinikum Heidelberg , Heidelberg,and 4d Nationales Centrum für Tumorerkrankungen , Heidelberg, Cellules souches normales et cancéreuses, Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université Montpellier 1 (UM1), Hematology, Basic (bio-) Medical Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellular differentiation [SDV]Life Sciences [q-bio] Naive B cell Cell Plasma Cells Kruppel-Like Transcription Factors Bone Marrow Cells/cytology Bone Marrow Cells Plasma cell Biology Cell Line 03 medical and health sciences Kruppel-Like Factor 4 Cell Cycle News & Views stomatognathic system Report medicine Humans Genetics(clinical) Molecular Biology B cell ComputingMilieux_MISCELLANEOUS [SDV.GEN]Life Sciences [q-bio]/Genetics CD40 hematology Caspases/metabolism Gene Expression Profiling Cell Differentiation Cell Biology Cell biology Plasma Cells/cytology 030104 developmental biology medicine.anatomical_structure KLF4 Apoptosis Caspases Immunology biology.protein Developmental Biology Kruppel-Like Transcription Factors/metabolism |
| Zdroj: | Cell Cycle Cell Cycle, Taylor & Francis, 2016, 15 (14), pp.1919-1928. ⟨10.1080/15384101.2016.1191709⟩ |
| ISSN: | 1538-4101 1551-4005 |
| DOI: | 10.1080/15384101.2016.1191709⟩ |
| Popis: | A role of the transcription factor Kruppel-like factor 4 (KLF4) in the generation of mature plasma cells (PC) is unknown. Indeed, KLF4 is critical in controlling the differentiation of various cell linages, particularly monocytes and epithelial cells. KLF4 is expressed at low levels in pro-B cells and its expression increases as they mature into pre-B cells, resting naive B cells and memory B cells. We show here that KLF4 is expressed in human bone marrow plasma cells and its function was studied using an in vitro model of differentiation of memory B cells into long lived plasma cells. KLF4 is rapidly lost when memory B cells differentiate into highly cell cycling plasmablasts, poorly cycling early plasma cells and then quiescent long-lived plasma cells. A forced expression of KLF4 in plasmablasts enhances the yield of their differentiation into early plasma cell and long lived plasma cells, by inhibiting apoptosis and upregulating previously unknown plasma cell pathways. |
| Databáze: | OpenAIRE |
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