Overexpression of MinE gene affects the plastid division in cassava
| Autor: | Xiaohan Liu, Lei Ke, Yi Min, Congcong Wang, Yu Xiao, Yuan Yao, Liang-Jing Cao, Peng Cao, Jianchun Guo, Ying Wang, Meng-Ting Geng, Wu Huang |
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| Rok vydání: | 2019 |
| Předmět: |
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Molecular 0106 biological sciences 0301 basic medicine Manihot Protein Conformation Cell Biology medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Genome Analytical Chemistry 03 medical and health sciences Gene Expression Regulation Plant medicine Amino Acid Sequence Plastids Plastid Molecular Biology Gene Escherichia coli Plant Proteins fungi Organic Chemistry food and beverages General Medicine Protoplast Subcellular localization Cell biology Chloroplast 030104 developmental biology medicine.anatomical_structure 010606 plant biology & botany Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 83:95-105 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2018.1518703 |
| Popis: | The MinE protein plays an important role in plastid division. In this study, the MinE gene was isolated from the cassava (Manihot esculenta Crantz) genome. We isolated high quality and quantity protoplasts and succeed in performing the transient expression of the GFP-fused Manihot esculenta MinE (MeMinE) protein in cassava mesophyll protoplasts. The transient expression of MeMinE-GFP in cassava protoplasts showed that the MeMinE protein was located in the chloroplast. Due to the abnormal division of chloroplasts, overexpression of MeMinE proteins in cassava mesophyll protoplasts could result in fewer and smaller chloroplasts. Overexpression of MeMinE proteins also showed abnormal cell division characteristics and minicell occurrence in Escherichia coli caused by aberrant septation events in the cell poles. MeMinE proteins function in cassava chloroplasts and Escherichia coli. The MinE protein plays an important role in plastid division. |
| Databáze: | OpenAIRE |
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