Evaluation of autoantibodies against vimentin and α-enolase in rheumatoid arthritis patients
| Autor: | Mina Ebrahimi-Rad, Mahdi Mahmoudi, Alireza Jamshidi, Hamid Mahmoudzadeh-Niknam, H Akhbari, Shohreh Khatami, Farhad Riazi-Rad, Reza Saghiri, Shirin Valadbeigi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
rheumatoid arthritis
medicine.medical_specialty autoantibodies Immunology Population Enolase Gastroenterology disease activity score 03 medical and health sciences 0302 clinical medicine vimentin synovial fluid Rheumatology anti-citrullinated peptide antibodies Internal medicine medicine α-enolase Immunology and Allergy Synovial fluid Rheumatoid factor education 030203 arthritis & rheumatology education.field_of_study Original Paper medicine.diagnostic_test business.industry Autoantibody medicine.disease Disease Activity Score Erythrocyte sedimentation rate Rheumatoid arthritis -enolase Medicine business |
| Zdroj: | Rheumatology, Vol 58, Iss 6, Pp 350-356 (2020) Reumatologia |
| ISSN: | 2084-9834 0034-6233 |
| Popis: | IntroductionRheumatoid arthritis (RA) is categorized as an autoimmune disease with a frequency of 0.2–1% worldwide. It is reported that various autoantibodies are produced in the RA population, particularly against citrullinated peptides. Among various candidate markers for RA diagnosis, the citrullinated proteins have the highest specificity and sensitivity for both diagnosis and prognosis of RA. Anti-mutated citrullinated vimentin and -enolase constitute a new class of autoantibodies for early detection of RA.Material and methods45 serum samples and 19 synovial fluid (SF) specimens collected from RA patients were considered for American College of Rheumatology criteria and 20 serum samples and 10 SF specimens were provided from healthy subjects as a control group. To assess the quantity of anti-citrullinated protein antibodies (ACPA), anti-mutated citrullinated vimentin (MCV) and anti--enolase in the serum and SF of RA patients were determined by the enzyme-linked immunosorbent assay (ELISA) method. For the evaluation of disease activity and joint destruction, we used the Disease Activity Score of 28 joints based on erythrocyte sedimentation rate (ESR) Disease Activity Score 28 (DAS28). Furthermore, to measure the molecular weight of vimentin and -enolase, electrophoresis on 10% SDS-PAGE was performed as described before.ResultsThe anti--enolase level among serum samples from RA patients was significantly higher than in healthy subjects (4.49 ±0.20 ng/ml vs. 0.76 ±0.12 ng/ml) (p < 0.001). There was a direct relation between -enolase quantity and (rheumatoid factor) RF and C-reactive protein (CRP) levels. The mean ESR value in positive and negative ACPA patients was 38.2 ±22.6 mm/h and 9.2 ±5.8 mm/h respectively (p < 0.0001). The mean DAS28-ESR was 3.3. The level of anti-MCV in the serum of RA patients (244.6 ±53.3 U/ml) was higher than in serum of the healthy group (148.73 ±71.8) (p < 0.0001). The level of anti-MCV in the SF of patients was 687.5 ±148.4 U/ml.ConclusionsIn conclusion, both autoantibodies against MCV and -enolase are two important markers that increase in serum and SF of RA patients and are specific for diagnosis of RA disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|