Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel
| Autor: | Bettina Bradatsch, Bettina Böttcher, Sander Granneman, David Tollervey, Ed Hurt, Christoph Leidig, Roland Beckmann, Marén Gnädig |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
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Molecular Saccharomyces cerevisiae Proteins Biology Ribosome Article 03 medical and health sciences 0302 clinical medicine Structural Biology Ribosomal protein medicine Eukaryotic Small Ribosomal Subunit Nuclear export signal Molecular Biology 030304 developmental biology Cell Nucleus 0303 health sciences Eukaryotic Large Ribosomal Subunit Cryoelectron Microscopy Biological Transport Cell nucleus Crystallography medicine.anatomical_structure Biophysics Eukaryotic Ribosome Nucleus Ribosomes 030217 neurology & neurosurgery |
| Zdroj: | Nature structural & molecular biology Bradatsch, B, Leidig, C, Granneman, S, Gnädig, M, Tollervey, D, Böttcher, B, Beckmann, R & Hurt, E 2012, ' Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel ', Nature Structural & Molecular Biology . https://doi.org/10.1038/nsmb.2438 |
| ISSN: | 1545-9985 1545-9993 |
| DOI: | 10.1038/nsmb.2438 |
| Popis: | Pre-ribosomal particles evolve in the nucleus through transient interaction with biogenesis factors, before export to the cytoplasm. Here, we report the architecture of the late pre-60S particle purified from Saccharomyces cerevisiae through Arx1, a nuclear export factor with structural homology to methionine aminopeptidases, or its binding partner Alb1. Cryo-electron microscopy reconstruction of the Arx1-particle at 11.9 Å resolution reveals regions of extra densities on the pre-60S particle attributed to associated biogenesis factors, confirming the immature state of the nascent subunit. One of these densities could be unambiguously assigned to Arx1. Immuno-electron microscopy and UV cross-linking localize Arx1 close to the ribosomal exit tunnel in direct contact with ES27, a highly dynamic eukaryotic rRNA expansion segment. The binding of Arx1 at the exit tunnel may position this export factor to prevent premature recruitment of ribosome-associated factors active during translation. |
| Databáze: | OpenAIRE |
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