Dietary modulation of the gut microbiota - a randomised controlled trial in obese postmenopausal women
| Autor: | Lena K. Brahe, Emmanuelle Le Chatelier, Edi Prifti, Nicolas Pons, Sean Kennedy, Trine Blædel, Janet Håkansson, Trine Kastrup Dalsgaard, Torben Hansen, Oluf Pedersen, Arne Astrup, S. Dusko Ehrlich, Lesli H. Larsen |
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| Přispěvatelé: | Department of Nutrition, Exercise and Sports [Copenhagen], Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), MetaGenoPolis, Institut National de la Recherche Agronomique (INRA), University of Copenhagen = Københavns Universitet (KU), Arla Strategic Innovation Centre [Sweden], Aarhus University [Aarhus], Novo Nordisk Foundation Center for Basic Metabolic Research (CBMR), Faculty of Health and Medical Sciences, The present study was financially supported by The Lundbeck Foundation Centre for Applied Medical Genomics in Personalized Disease Prediction, Prevention and Care (http://www.LuCAMP.org), and The Danish Dairy Board. Arla Foods amba delivered the probiotic product and provided additional funding. Metagenopolis is funded by the Investissement d'Avenir grant ANR-11-DPBS-0001., The authors thank the study participants, the kitchen staff, dieticians, laboratory technicians, good clinical practice coordinator and students involved in the project and the staff from the INRA/MetaGenoPolis: Florence Levenez and Joël Doré for sample preparation, Nathalie Galleron and Benoit Quinquis for microbiome sequencing, Jean-Michel Batto and Pierre Leonard for informatics. In addition to Sander Kersten, Division of Human Nutrition, Wageningen University, who kindly provided the protocol and guidance for the ANGPTL4 analyses. The study is part of The Lundbeck Foundation Centre for Applied Medical Genomics in Personalized Disease Prediction, Prevention and Care (http://www.LuCAMP.org)., ANR-11-DPBS-0001,MGP,MetaGenoPolis(2011), Brahe, Lena K., Le Chatelier, Emmanuelle |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
MESH: C-Peptide/blood
medicine.medical_treatment MESH: Probiotics/therapeutic use Medicine (miscellaneous) Physiology MESH: Insulin Secretion Gut flora Feces chemistry.chemical_compound Flax Lactobacillus Insulin Secretion MESH: Intestines/microbiology Insulin Single-Blind Method MESH: Aged 2. Zero hunger Glucose tolerance test MESH: Middle Aged Nutrition and Dietetics C-Peptide medicine.diagnostic_test C-peptide MESH: Feces/microbiology Full Papers Middle Aged 3. Good health Intestines Postmenopause obésité sensibilité à l'insuline MESH: Insulin Resistance Plant Mucilage [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MESH: Obesity/microbiology femme menopausée MESH: Obesity/complications Female Flaxseed mucilage MESH: Plant Mucilage/administration & dosage Obesity-related disease MESH: Diet Human and Clinical Nutrition lactobacillus paracasei MESH: Glucose Tolerance Test Gut microbiota Biology digestive system Microbiology Insulin resistance microbiote medicine Humans Obesity Aged Probiotics Metagenomics MESH: Insulin/metabolism MESH: Humans MESH: Obesity/diet therapy Glucose Tolerance Test MESH: Single-Blind Method medicine.disease biology.organism_classification Diet MESH: Flax MESH: Prebiotics Prebiotics chemistry Insulin Resistance MESH: Postmenopause graine de lin MESH: Lactobacillus MESH: Female [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | Brahe, L K, Le Chatelier, E, Prifti, E, Pons, N, Kennedy, S, Blædel, T, Håkansson, J, Dalsgaard, T K, Hansen, T, Pedersen, O B, Astrup, A, Ehrlich, S D & Larsen, L H 2015, ' Dietary modulation of the gut microbiota-a randomised controlled trial in obese postmenopausal women ', British Journal of Nutrition, vol. 114, no. 3, pp. 406-417 . https://doi.org/10.1017/S0007114515001786 British Journal of Nutrition British Journal of Nutrition, Cambridge University Press (CUP), 2015, 114 (3), pp.406-417. ⟨10.1017/S0007114515001786⟩ British Journal of Nutrition 3 (114), 406-417. (2015) The British Journal of Nutrition |
| ISSN: | 0007-1145 1475-2662 |
| DOI: | 10.1017/S0007114515001786 |
| Popis: | The gut microbiota has been implicated in obesity and its progression towards metabolic disease. Dietary interventions that target the gut microbiota have been suggested to improve metabolic health. The aim of the present study was to investigate the effect of interventions withLactobacillus paracaseiF19 or flaxseed mucilage on the gut microbiota and metabolic risk markers in obesity. A total of fifty-eight obese postmenopausal women were randomised to a single-blinded, parallel-group intervention of 6-week duration, with a daily intake of eitherL. paracaseiF19 (9·4 × 1010colony-forming units), flaxseed mucilage (10 g) or placebo. Quantitative metagenomic analysis of faecal DNA was performed to identify the changes in the gut microbiota. Diet-induced changes in metabolic markers were explored using adjusted linear regression models. The intake of flaxseed mucilage over 6 weeks led to a reduction in serum C-peptide and insulin release during an oral glucose tolerance test (PPPFaecalibacteriumspecies. These changes in the microbiota could not explain the effect of flaxseed mucilage on insulin sensitivity. The intake ofL. paracaseiF19 did not modulate metabolic markers compared with placebo. In conclusion, flaxseed mucilage improves insulin sensitivity and alters the gut microbiota; however, the improvement in insulin sensitivity was not mediated by the observed changes in relative abundance of bacterial species. |
| Databáze: | OpenAIRE |
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