Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance
| Autor: | Ivana Strahinic, Geert Huys, Ana Belén Flórez, Ljubisa Topisirovic, Klaas D'Haene, Baltasar Mayo, Milan Kojic, Jelena Lozo, Jelena Begovic |
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| Přispěvatelé: | Ministry of Education, Science and Technological Development (Serbia), European Commission, Research Foundation - Flanders |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Vaginal lactobacilli Molecular Sequence Data Erythromycin Microbial Sensitivity Tests Erythromycin resistance DNA Ribosomal Microbiology 03 medical and health sciences Antibiotic resistance Lactobacillus rhamnosus 23S ribosomal RNA Drug Resistance Bacterial medicine Humans Molecular Biology 030304 developmental biology Antibacterial agent Genetics 0303 health sciences Base Sequence biology Lacticaseibacillus rhamnosus 030306 microbiology 23S rRNA General Medicine biology.organism_classification Anti-Bacterial Agents 3. Good health Mutational analysis RNA Ribosomal 23S Mutation Vagina Female Christian ministry medicine.drug |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0923-2508 |
| Popis: | Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A → G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 μg mL-1). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli. © 2009 Elsevier Masson SAS. All rights reserved. This work was supported by the Ministry of Science and Technological Development, Republic of Serbia, Grant No 143036 and by a EU project within the VI Frame Program (ACE-ART, ref. CT-2003-506214). G.H. is a postdoctoral fellow of the Fund for Scientific Research, Flanders (Belgium) (F.W.O.-Vlaanderen). |
| Databáze: | OpenAIRE |
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