Molecular Cloning and Expression Analysis of Cryptochrome Gene PsCRY2 in Tree Peony
| Autor: | Xiuxin Zhang, Shunli Wang, Jingqi Xue, Fuyong Zhu, Chuanjiao Liu, Xiuxia Ren |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Science lcsh:Plant culture Biology Molecular cloning 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences Cryptochrome photoperiod pathway Arabidopsis Botany Gene expression lcsh:SB1-1110 PsCRY2 Gene Ecology Evolution Behavior and Systematics photoperiodism Ecology Renewable Energy Sustainability and the Environment Paeonia suffruticosa food and beverages qRT-PCR Herbaceous plant biology.organism_classification cryptochromes tree peony 030104 developmental biology 010606 plant biology & botany |
| Zdroj: | Horticultural Plant Journal, Vol 2, Iss 6, Pp 357-362 (2016) |
| ISSN: | 2468-0141 |
| DOI: | 10.1016/j.hpj.2016.10.003 |
| Popis: | Cryptochromes are blue/ultraviolet-A (UV-A) light receptors involved in regulating various aspects of plant growth and development. Investigations of the structure and functions of cryptochromes in plants have largely focused on herbaceous plants. However, few data on the function of CRY2 are available in woody plants. In this study, a cryptochrome 2 (CRY2) gene was isolated from Paeonia suffruticosa by Reverse Transcription Polymerase Chain Reaction (RT-PCR). Sequence alignment and motif analysis showed that the deduced amino acids contained a PHR domain near the amino terminus and a CCT domain near the carboxy terminus. PsCRY2 showed high identity with AtCRY2 of Arabidopsis. Phylogenetic analysis indicated that it was closely related to Citrus sinensis. Gene expression analysis revealed that the highest expression levels of PsCRY2 occurred in the bud and seed embryo of P. suffruticosa, followed by the roots, stems, and leaves. PsCRY2 was upregulated during the entire process of bud differentiation, whereas this was downregulated during the early stage of bud development and upregulated in the middle and late stages. The highest level of PsCRY2 expression was observed in the big bell-like flower buds. These results suggested that PsCRY2 plays an important role in both bud differentiation and bud development. The expression patterns of PsCRY2 in the buds of ‘Luoyanghong’ and ‘Qiufa 1’ were similar, whereas that in the buds of ‘Qiufa 1’ was significantly higher than in the buds of ‘Luoyanghong’. The buds of plants subjected to different photoperiod treatments exhibited variations in PsCRY2 expression patterns. The expression of PsCRY2 decreased during bud sprouting and in the small bell-like flower buds that were subjected to short-day photoperiod compared to that observed under long-day photoperiod. |
| Databáze: | OpenAIRE |
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