Polymorphonuclear leukocyte apoptosis is accelerated by sulfatides or sulfatides-treated Salmonella Typhimurium bacteria
| Autor: | Yulia M. Romanova, Galina F. Sud'ina, Zoryana V. Grishina, Galina M. Viryasova |
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| Rok vydání: | 2014 |
| Předmět: |
Salmonella typhimurium
Ceramide Article Subject Neutrophils Phagocytosis Population lcsh:Medicine Apoptosis Ceramides General Biochemistry Genetics and Molecular Biology Microbiology Flow cytometry chemistry.chemical_compound medicine Humans education Opsonin Caspase education.field_of_study Sulfoglycosphingolipids General Immunology and Microbiology medicine.diagnostic_test biology lcsh:R General Medicine Opsonin Proteins Flow Cytometry beta-Galactosidase chemistry Caspases biology.protein Intracellular Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2015 (2015) |
| ISSN: | 2314-6141 |
| Popis: | Neutrophils die by apoptosis following activation and uptake of microbes or enter apoptosis spontaneously at the end of their lifespan if they do not encounter a pathogen. Here we report that sulfatides or sulfatides-treatedSalmonellaTyphimurium bacteria accelerated human neutrophil apoptosis. Neutrophil apoptosis was examined by flow cytometry. Sulfatides caused prominent increase in percentage of apoptotic cells after 2.5 hrs of incubation.SalmonellaTyphimurium bacteria by themselves did not affect the basal level of apoptosis in neutrophil population. When neutrophils were added toS.Typhimurium “opsonized” by sulfatides, apoptotic index significantly increased, whereas the number of phagocyting cells was not influenced. Sulfatides’ proapoptotic effect was strongly dependent on the activity ofβ-galactosidase; inhibition of this enzyme impaired its potency to accelerate apoptosis. These data support the mechanism of neutrophil apoptosis triggering based on sulfatides’ ability to accumulate in intracellular compartments and mediate successive increase in ceramide content resulting fromβ-galactosidase activity. |
| Databáze: | OpenAIRE |
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