Development, validation and clinical application of an online-SPE-LC-HRMS/MS for simultaneous quantification of phenobarbital, phenytoin, carbamazepine, and its active metabolite carbamazepine 10,11-epoxide
| Autor: | Zheng Yin, Kai Ma, Yuanjie Fan, Lihua Qu, Wenjun Wang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Phenytoin Spectrometry Mass Electrospray Ionization Bioanalysis Epoxide 02 engineering and technology 01 natural sciences Analytical Chemistry chemistry.chemical_compound Tandem Mass Spectrometry medicine Humans Solid phase extraction Chromatography High Pressure Liquid Active metabolite Epilepsy Chromatography medicine.diagnostic_test Solid Phase Extraction 010401 analytical chemistry Reproducibility of Results Carbamazepine 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Therapeutic drug monitoring Phenobarbital Anticonvulsants Female Drug Monitoring 0210 nano-technology medicine.drug |
| Zdroj: | Talanta. 158:77-88 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2016.05.036 |
| Popis: | A simple and efficient bioanalytical method for simultaneous determination of phenobarbital (PB), phenytoin (PHT), carbamazepine (CBZ), and its active metabolite carbamazepine 10,11-epoxide (CBZE) in human plasma using online solid phase extraction (SPE)-liquid chromatography (LC) coupled with high resolution mass spectrum (HRMS) under targeted MS/MS (t-MS(2)) analysis mode has been developed. The procedure integrated an automated sample clean-up of human plasma by Oasis®HLB SPE cartridge, a separation by ZORBAX SB-C18 analysis column, and a quantification by Q-Exactive Hybrid Quadrupole-Orbitrap. The total running time was 13min. The lower limit of quantification (LLOQ) of PB, PHT, CBZ, and CBZE were 0.008, 0.008, 0.0016 and 0.0016μgmL(-1) respectively and the linearities were in the range of 0.008-2.500, 0.008-2.500, 0.0016-0.500 and 0.0016-0.500μgmL(-1) respectively. The mean recovery was between 91.82% and 108.27% and the matrix effect was between 93.29% and 102.09%. The relative standard deviations of interday and intraday were less than 6.41%. The method has been successfully applied in therapeutic drug monitoring (TDM) of four Chinese epilepsy patients. This fully automated, simple, sensitive and reliable online-SPE-LC-HRMS/MS method serves well for TDM of PB, PHT, CBZ and CBZE at clinics for either single or combination treatment. |
| Databáze: | OpenAIRE |
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