The morphology of cytoplasts and karyoplasts produced by cytochalasin D

Autor: L. Skinnider
Rok vydání: 1979
Předmět:
Zdroj: Experientia. 35:426-427
ISSN: 1420-9071
0014-4754
DOI: 10.1007/bf01964390
Popis: The denucleation of L cells and human lymphocytes in suspension was carried out by incubation with cytochalasin D25 and 50 microgram/ml and with ultracentrifugation over a discontinuous Ficoll gradient. Ultrastructural examination of the separate layers confirmed the presence of the anucleate cytoplasm (cytoplasts) with intact membrane and the separated nuclei (karyoplasts).
Databáze: OpenAIRE