Capsaicin and dihydrocapsaicin induce apoptosis in human glioma cells via ROS and Ca2+-mediated mitochondrial pathway
| Autor: | You‑Ren Zhang, Le Xie, Lingyun Zhao, Jintian Tang, Shen Zhou, Guang‑Hong Xiang, Tao Tang, Dong Liu, Yan Tang, Da‑Hua Wu |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Biology Biochemistry capsaicin dihydrocapsaicin Flow cytometry 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Glioma Cell Line Tumor Genetics medicine Animals Humans Viability assay Calcium Signaling Molecular Biology Cell Proliferation medicine.diagnostic_test apoptosis Articles Cell cycle medicine.disease Xenograft Model Antitumor Assays mitochondrial pathway Mitochondria 030104 developmental biology medicine.anatomical_structure Oncology chemistry Capsaicin Apoptosis Cell culture 030220 oncology & carcinogenesis Cancer research Molecular Medicine Neoplasm Recurrence Local Capsicum Reactive Oxygen Species |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Human glioma is the most common type of primary brain tumor and one of the most invasive and aggressive tumors, which, even with treatments including surgery, radiotherapy and chemotherapy, often relapses and exhibits resistance to conventional treatment methods. Developing novel strategies to control human glioma is, therefore, an important research focus. The present study investigated the mechanism of apoptosis induction in U251 human glioma cells by capsaicin (Cap) and dihydrocapsaicin (DHC), the major pungent ingredients of red chili pepper, using the Cell Counting Kit‑8 assay, transmission electron microscopy analysis, flow cytometry analysis, laser scanning confocal microscope analysis and immunohistochemical staining. Treatment of U251 glioma cells with Cap and DHC resulted in a dose‑ and time‑dependent inhibition of cell viability and induction of apoptosis, whereas few effects were observed on the viability of L929 normal murine fibroblast cells. The apoptosis‑inducing effects of Cap and DHC in U251 cells were associated with the generation of reactive oxygen species, increased Ca2+ concentrations, mitochondrial depolarization, release of cytochrome c into the cytosol and activation of caspase‑9 and ‑3. These effects were further confirmed by observations of the anti‑tumor effects of Cap and DHC in vivo in a U251 cell murine tumor xenograft model. These results demonstrate that Cap and DHC are effective inhibitors of in vitro and in vivo survival of human glioma cells, and provide the rationale for further clinical investigation of Cap and DHC as treatments for human glioma. |
| Databáze: | OpenAIRE |
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