Role of the ATP-dependent chromatin remodeling enzyme Fun30/Smarcad1 in the regulation of mRNA splicing
| Autor: | Bo-Shiun Chen, Wei Wang, Qiankun Niu, Asim Bikas Das, Wei Hua Wu, Boseon Byeon, Zhe Wei |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Spliceosome Saccharomyces cerevisiae Proteins Heterochromatin RNA Splicing ATP-dependent chromatin remodeling Biophysics Saccharomyces cerevisiae Biology Biochemistry Chromatin remodeling Article 03 medical and health sciences Mice 0302 clinical medicine Adenosine Triphosphate Animals RNA Messenger Molecular Biology Gene Alternative splicing DNA Helicases Cell Biology Chromatin Cell biology Mice Inbred C57BL 030104 developmental biology 030220 oncology & carcinogenesis RNA splicing Transcription Factors |
| Zdroj: | Biochem Biophys Res Commun |
| ISSN: | 1090-2104 |
| Popis: | The yeast ATP-dependent chromatin remodeling enzyme Fun30 has been shown to regulate heterochromatin silencing, DNA repair, transcription, and chromatin organization. Although chromatin structure has been proposed to influence splice site recognition and regulation, whether ATP-dependent chromatin remodeling enzyme plays a role in regulating splicing is not known. In this study, we find that pre-mRNA splicing efficiency is impaired and the recruitment of spliceosome is compromised in Fun30-depleted cells. In addition, Fun30 is enriched in the gene body of individual intron-containing genes. Moreover, we show that pre-mRNA splicing efficiency is dependent on the chromatin remodeling activity of Fun30. The function of Fun30 in splicing is further supported by the observation that, Smarcad1, the mammalian homolog of Fun30, regulates alternative splicing. Taken together, these results provide evidence for a novel role of Fun30 in regulating splicing. |
| Databáze: | OpenAIRE |
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