Effects of etanercept, a tumor necrosis factor receptor fusion protein, on primary cell cultures prepared from intact human intervertebral disc tissue

Autor: Ibrahim Yilmaz, Ozkan Ates, Hanefi Ozbek, Tezcan Caliskan, Yasin Emre Kaya, Abdullah Talha Simsek, Numan Karaarslan, Yener Akyuva, Necati Kaplan, Duygu Yasar Sirin, Aliye Yildirim Guzelant
Přispěvatelé: BAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Kaya, Yasin Emre
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Experimental and Therapeutic Medicine
ISSN: 1792-1015
1792-0981
Popis: WOS:000476606000009 PubMed: 31258639 The aim of the present study was to investigate the effects of etanercept (ETA), a tumor necrosis factor (TNF) inhibitor, on human cell cultures prepared from intact intervertebral disc tissue. ETA is used as a treatment for cases of rheumatoid arthritis, psoriatic arthritis, axial spondyloarthritis and ankylosing spondylitis accompanied by moderate or severe joint pain. ETA was applied to primary cell cultures [annulus fibrosus and nucleus pulposus (NP) from intact intervertebral disc tissue]. Cell cultures without ETA treatment served as the control group. Morphological and quantitative molecular analyses of the two groups were performed. The number of viable cells and cell proliferation decreased in the ETA-treated cultures as compared with those in the control group. Furthermore, in the treatment group, the chondroadherin gene, an NP-specific marker, was not expressed after 24 h. By contrast, the cartilage oligo matrix protein was expressed 24, 48 and 72 h post-ETA treatment, while its expression was significantly lower than that in the control group. In addition, the expression of interleukin-1 beta, as well as matrix metallopeptidase-7 and -19, was markedly decreased. Overall, the cell proliferation and gene expression in the ETA-treated cells were significantly different from those in the control group (P
Databáze: OpenAIRE
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