An activator-induced quencher-detachment-based turn-on probe with a cationic substrate moiety for acetylcholinesterase
| Autor: | Masahiro Oe, Kouichi Ohe, Akito Masuda, Koji Miki, Kohei Nogita |
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| Rok vydání: | 2021 |
| Předmět: |
Fluorophore
Cell Survival PC12 Cells Catalysis Substrate Specificity chemistry.chemical_compound Polymer chemistry Materials Chemistry Moiety Animals Carboxylate Fluorescent Dyes Microscopy Confocal Activator (genetics) Hydrolysis Metals and Alloys Cationic polymerization Substrate (chemistry) General Chemistry Carbocyanines Acetylcholinesterase Fluorescence Surfaces Coatings and Films Electronic Optical and Magnetic Materials Rats Spectrometry Fluorescence chemistry Ceramics and Composites Cholinesterase Inhibitors |
| Zdroj: | Chemical communications (Cambridge, England). 58(10) |
| ISSN: | 1364-548X |
| Popis: | We report a choline ester-grafted turn-on fluorescence probe to detect acetylcholinesterase (AChE) in living cells. The AChE-mediated hydrolysis of the choline ester moiety producing carboxylate initiates the activation of the Cy5 fluorophore quenched by an intramolecular nucleophilic mercapto group. The probe has the advantages of high AChE affinity and low cytotoxicity. |
| Databáze: | OpenAIRE |
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