Detection of Opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes by a PCR-based method
| Autor: | Wilawan Pumidonming, Phunthira Pongsaskulchoti, Wanchai Maleewong, T. Kowsuwan, V. Kitikoon, Chaisiri Wongkham, Thitima Wongsaroj, Pewpan M. Intapan |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Fasciola gigantica Snails Cyprinidae Zoology Snail Opisthorchiasis Polymerase Chain Reaction Sensitivity and Specificity Fish Diseases Cricetinae biology.animal Gastropoda Animals Parasite hosting Opisthorchis viverrini biology Ecology Opisthorchis Intermediate host DNA Helminth biology.organism_classification Infectious Diseases Female Animal Science and Zoology Parasitology Trematoda Haplorchis taichui |
| Zdroj: | Parasitology. 126:63-67 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182002002573 |
| Popis: | A PCR procedure for the detection of Opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes was developed. This procedure was based on primers designed from a pOV-A6 specific probe sequence giving a 330 base-pair product. The detection was accomplished in host tissue homogenates to which a single cercaria or metacercaria was introduced. PCR can detect as little as a single cercaria artificially inoculated in a snail or a single metacercaria artificially inoculated in a fish sample. The method gave a 100% positivity rate for all infected snails or fishes. The method did not yield a 330 base-pair amplified product with other digenean fluke DNAs such as Haplorchis taichui, Centrocestus spp., Echinostoma malayanum, Fasciola gigantica, animal schistosomes, Paragonimus heterotremus or Haplorchoides spp. The assay has great potential for application in epidemiological surveys of both snail and fish intermediate hosts as well as for investigation of foodborne parasites in freshwater fishes. |
| Databáze: | OpenAIRE |
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