Evaluation of 3 Serological Tests for Early Detection Of Leptospira‐specific Antibodies in Experimentally Infected Dogs
| Autor: | Patrick F.M. Meeus, Joshua T. Lizer, A. Weber, Matthew R. Krecic, Sridhar Velineni |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Serotype Male IgM 040301 veterinary sciences Immunology Standard Article Serology 0403 veterinary science 03 medical and health sciences Dogs Antigen Leptospira Direct agglutination test medicine SNAP Lepto Animals Leptospirosis Serologic Tests Dog Diseases Seroconversion General Veterinary biology business.industry Microscopic agglutination test WITNESS Lepto 04 agricultural and veterinary sciences medicine.disease biology.organism_classification Virology Antibodies Bacterial Standard Articles 030104 developmental biology Early Diagnosis biology.protein SMALL ANIMAL Antibody business Canine leptospirosis |
| Zdroj: | Journal of Veterinary Internal Medicine |
| ISSN: | 1939-1676 0891-6640 |
| Popis: | Background Leptospirosis in dogs is a disease of global importance. Early detection and appropriate therapeutic intervention are necessary to resolve infection and prevent zoonotic transmission. However, its diagnosis is hindered by nonspecific clinical signs and lack of rapid diagnostic tests of early infection. Recently, 2 rapid point-of-care tests (WITNESS Lepto [WITNESS Lepto, Zoetis LLC, Kalamazoo, MI, USA] and SNAP Lepto [SNAP Lepto, IDEXX Laboratories, Westbrook, ME, USA]) for detection of Leptospira-specific antibodies in canine sera were developed. Hypothesis Immunoglobulin M-based WITNESS Lepto containing multiple detection antigens can detect Leptospira-specific antibodies to common leptospiral serovars earlier in the course of infection as compared to microscopic agglutination test (MAT) and SNAP Lepto. Animals Four groups of 8 6- to 8-month-old male Beagle dogs were used. Methods Thirty-two healthy seronegative dogs were inoculated experimentally with serovars Canicola, Grippotyphosa, Icterohaemorrhagiae, and Pomona (8 dogs/serovar). Acute-phase sera were collected at regular intervals and monitored for Leptospira-specific antibodies by WITNESS Lepto, MAT, and SNAP Lepto. Results Seroconversion was detected in all dogs by day 10 by WITNESS Lepto and in 30 of 32 dogs by day 14 by MAT. The SNAP Lepto test detected seroconversion in 3 dogs during the 2 weeks postchallenge. Conclusions Immunoglobulin M-based WITNESS Lepto detected immune responses specific to multiple leptospiral serovars early in the course of infection and identified seroconversion in all animals earlier than did the gold standard MAT. The SNAP Lepto test displayed considerably lower and inconsistent performance during the study period. At the point-of-care, WITNESS Lepto should be the test of choice for rapid and reliable screening of acutely ill dogs suspected to have leptospirosis. |
| Databáze: | OpenAIRE |
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