Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dür)
| Autor: | Hang-Wei Liu, Yu-Yuan Guo, Yong-Jun Zhang, Liang Sun, Dan-feng Liu, Xingkui An, Yu-Xiao Ding, Le-Mei Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Arthropod Antennae
Male 0106 biological sciences 0301 basic medicine Real-Time Polymerase Chain Reaction Receptors Odorant 01 natural sciences Article Heteroptera Transcriptome 03 medical and health sciences Botany medicine Animals Gene family Apolygus lucorum Gene Phylogeny Genetics Cloning Multidisciplinary Olfactory receptor biology Phylogenetic tree Gene Expression Profiling biology.organism_classification 010602 entomology 030104 developmental biology medicine.anatomical_structure Multigene Family Sex pheromone Insect Proteins Female |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep37870 |
| Popis: | Olfactory receptors are believed to play a central role in insects host-seeking, mating, and ovipositing. On the basis of male and female antennal transcriptome of adult Apolygus lucorum, a total of 110 candidate A. lucorum odorant receptors (AlucOR) were identified in this study including five previously annotated AlucORs. All the sequences were validated by cloning and sequencing. Tissue expression profiles analysis by RT-PCR indicated most AlucORs were antennal highly expressed genes. The qPCR measurements further revealed 40 AlucORs were significantly higher in the antennae. One AlucOR was primarily expressed in the female antennae, while nine AlucORs exhibited male-biased expression patterns. Additionally, both the RPKM value and RT-qPCR analysis showed AlucOR83 and AlucOR21 were much higher abundant in male antennae than in female antennae, suggesting their different roles in chemoreception of gender. Phylogenetic analysis of ORs from several Hemipteran species demonstrated that most AlucORs had orthologous genes, and five AlucOR-specific clades were defined. In addition, a sub-clade of potential male-based sex pheromone receptors were also identified in the phylogenetic tree of AlucORs. Our results will facilitate the functional studies of AlucORs, and thereby provide a foundation for novel pest management approaches based on these genes. |
| Databáze: | OpenAIRE |
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