Development of an optical tyrosinase biosensor (TCA) for detection of 'Parathion-Methyl'
| Autor: | Fehime Cakıcıoglu Ozkan, İlker Polatoğlu |
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| Přispěvatelé: | Çakıcıoğlu Özkan, Fehime, Izmir Institute of Technology. Chemical Engineering, Department of Bioengineering, Manisa Celal Bayar University, Faculty of Engineering, Manisa, Turkey, Department of Chemical Engineering, Izmir Yuksek Teknoloji Enstitusu, Urla, Turkey |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Chromatography
Tyrosinase biosensor Tyrosinase Analytical technique 02 engineering and technology Parathion-methyl 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Industrial and Manufacturing Engineering Organophosphate pesticide 0104 chemical sciences Chitosan chemistry.chemical_compound Adsorption Non-competitive inhibition Biosensors chemistry Tap water Electrical and Electronic Engineering Fourier transform infrared spectroscopy 0210 nano-technology Biosensor |
| Popis: | Purpose This paper aims to present a novel and cost-effective optical biosensor design by simple preparation method for detection of “parathion-methyl,” which is a model pesticide pose to public health and the environment. Design/methodology/approach The optical enzyme biosensor (TCA) for detection of pesticide “parathion-methyl” was developed on the basis of immobilization of tyrosinase enzyme on chitosan film by adsorption technique. The analytic performance of TCA was investigated by measuring its activity with Ultraviolet (UV) visible spectrophotometer. Findings Uniform porous network structure and protonated groups of chitosan film provided a microenvironment for tyrosinase immobilization evident from Fourier transform infrared (FTIR) spectroscopy and Atomic Force Microscopy analysis. TCA has a wide linear detection range (0-1.03 µM) with high correlation coefficient and it can detect the parathion-methyl concentration as low as 159 nM by noncompetitive inhibition kinetics. Using the TCA sensor both for ten times and at least 45 days without a significant loss in its activity are the indicators of its good operational and storage stability. Moreover, TCA can be applicable to tap water, providing a promising tool for pesticides detection. Originality/value This is the first time to use the in situ analytical technique that can improve the performance of optical enzyme sensor provided to control the pesticide residue better with respect to traditional techniques. The effect of organic solvents on the performance of optical enzyme biosensor was investigated. Inhibition kinetic of the solvents rarely encountered in literature was also studied besides the pH and temperature tolerance of the optical biosensor. |
| Databáze: | OpenAIRE |
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