Removal of RNase activity from DNase by affinity chromatography on agarose coupled aminophenylphosphoryl-uridine-2' (3')-phosphate

Autor:	Ian H. Maxwell, Francoise Maxwell, William E. Hahn
Rok vydání:	1977
Předmět:	Deoxyribonucleases biology Uracil Nucleotides Sepharose RNA Molecular biology Uridine Chromatography Affinity chemistry.chemical_compound Ribonucleases Affinity chromatography chemistry Biochemistry Genetics biology.protein Agarose Pancreatic ribonuclease Incubation Uracil nucleotide
Zdroj:	Nucleic acids research. 4(1)
ISSN:	0305-1048
Popis:	Severe degradation of high molecular weight RNA was shown to occur during incubation with commercially purified DNase. Most of the RNase activity could be removed by passage of the DNase through a column of agarose-coupled amino phenylphosphoryl-uridine-2' (3')-phosphate. Incubation with the treated DNase caused only minimal alteration of the sedimentation pattern of high molecular weight nuclear RNA, determined under partially denturing conditions. No impairment of DNase activity was detected.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::deb73d84a42e80644fd9298b79d1af24 https://pubmed.ncbi.nlm.nih.gov/866176 Zobrazit plný text záznamu