Investigation of subcellular acidic compartments using alpha-aminophosphonate 31P nuclear magnetic resonance probes
| Autor: | Jean-Louis Clément, Sylvia Pietri, Michel Satre, Jean-Baptiste Martin, Bruno Blaive, Marcel Culcasi, Gaëlle Gosset |
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| Přispěvatelé: | Chimie, biologie et radicaux libres - UMR 6517 (CBRL), Université de la Méditerranée - Aix-Marseille 2-Université Paul Cézanne - Aix-Marseille 3-Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique (CNRS), Biochimie et biophysique des systèmes intégrés (BBSI), Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF), Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Biophysics
Organophosphonates [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biochemistry Dictyostelium discoideum Permeability 03 medical and health sciences 0302 clinical medicine Nuclear magnetic resonance Extracellular Animals Dictyostelium Amines [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Molecular Biology Nuclear Magnetic Resonance Biomolecular Cells Cultured 030304 developmental biology 0303 health sciences biology Chemistry Pinocytosis Myocardium Phosphorus Isotopes Heart Cell Biology Penetration (firestop) Permeation Hydrogen-Ion Concentration biology.organism_classification Rats Cytosol Aminophosphonate 030220 oncology & carcinogenesis Toxicity Neuroglia Subcellular Fractions |
| Zdroj: | Analytical Biochemistry Analytical Biochemistry, Elsevier Masson, 2008, 380 (2), pp.184-94. ⟨10.1016/j.ab.2008.05.052⟩ Analytical Biochemistry, 2008, 380 (2), pp.184-94. ⟨10.1016/j.ab.2008.05.052⟩ |
| ISSN: | 0003-2697 1096-0309 |
| DOI: | 10.1016/j.ab.2008.05.052⟩ |
| Popis: | The 31P nuclear magnetic resonance (NMR) characteristics, toxicity, and cellular penetration of five linear or cyclic alpha-aminophosphonate highly sensitive pH probes were investigated in Dictyostelium discoideum cells and isolated rat hearts and were compared with three phosphonic acid derivatives. The line width broadening at pH approximately pK(a), which was satisfactorily modelized for all compounds, was significantly limited in biological milieu for the new markers, affording a four- to sixfold better accuracy in pH determination. Cellular uptake or washout of nontoxic concentrations (< 15 mM) of alpha-aminophosphonates occurred by rapid passive permeation, whereas standard probes required a much slower fluid-phase pinocytosis and transport processes that could ultimately lead to trapping. Using mild concentrations (< 4 mM) three alpha-aminophosphonates having 6 < pK(a) < 7 allowed an easy and simultaneous 31P NMR determination of cytosolic, acidic, and extracellular compartments in anoxic-reoxygenated or starving D. discoideum. |
| Databáze: | OpenAIRE |
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