Noninvasive noncontact speckle contrast diffuse correlation tomography of cerebral blood flow in rats
Autor: | Elie G. Abu Jawdeh, Chong Huang, Siavash Mazdeyasna, Lei Chen, Li Chen, Kathryn E. Saatman, Henrietta S. Bada, Guoqiang Yu |
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Rok vydání: | 2019 |
Předmět: |
Male
Cognitive Neuroscience Partial volume Iterative reconstruction Article 050105 experimental psychology Brain Ischemia Rats Sprague-Dawley Correlation 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine medicine.artery medicine Animals 0501 psychology and cognitive sciences Common carotid artery Tomography Speckle contrast business.industry Optical Imaging 05 social sciences Brain Stroke medicine.anatomical_structure Neurology Cerebral blood flow Cerebrovascular Circulation Scalp business 030217 neurology & neurosurgery Biomedical engineering |
Zdroj: | Neuroimage |
ISSN: | 1053-8119 |
Popis: | Continuous and longitudinal imaging of cerebral blood flow (CBF) variations provide vital information to investigate pathophysiology and interventions for a variety of neurological and cerebral diseases. An innovative noncontact speckle contrast diffuse correlation tomography (scDCT) system was downscaled and adapted for noninvasive imaging of CBF distributions in rat brain through intact scalp and skull. Algorithms for 2D mapping and 3D image reconstruction of CBF distributions were developed and optimized. The continuous imaging capability of the system was shown by imaging global CBF increases during CO2 inhalations and regional CBF decreases across two hemispheres during sequential unilateral and bilateral common carotid artery ligations. The longitudinal imaging capability was demonstrated by imaging CBF variations over a long recovery period of 14 days after an acute stroke. Compared to the 2D mapping method, the 3D imaging method reduces partial volume effects, but needs more computation time for image reconstruction. Results from this study generally agree with those reported in the literature using similar protocols to induce CBF changes in rats. The scDCT enables a relatively large penetration depth (up to ∼10 mm), which is sufficient for transcranial brain measurements in small animals and human neonates. Ultimately, we expect to provide a noninvasive noncontact cerebral imager for basic neuroscience research in small animal models and clinical applications in human neonates. |
Databáze: | OpenAIRE |
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