Human Wharton's Jelly Mesenchymal Stem Cell-Mediated Sciatic Nerve Recovery Is Associated with the Upregulation of Regulatory T Cells
| Autor: | Aline Yen Ling Wang, Chin-Ming Lee, Chia-Hsien Lin, Sing-Ying Hsieh, Charles Yuen Yung Loh, Hsin-Hsin Shen, Ing-Kae Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Biology T-Lymphocytes Regulatory Catalysis Article regulatory T cells lcsh:Chemistry Inorganic Chemistry Neurotmesis 03 medical and health sciences Mice 0302 clinical medicine Neurotrophic factors Wharton's jelly Animals Immunologic Factors Wharton Jelly Physical and Theoretical Chemistry transection lcsh:QH301-705.5 Molecular Biology Spectroscopy Cells Cultured Cell Proliferation Mice Inbred BALB C Innate immune system Wharton’s jelly Organic Chemistry Mesenchymal stem cell Mesenchymal Stem Cells General Medicine Acquired immune system Sciatic Nerve Computer Science Applications Cell biology Treg 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 neurotmesis regeneration peripheral nerve Cytokines Sciatic nerve 030217 neurology & neurosurgery CD8 |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 17 International Journal of Molecular Sciences, Vol 21, Iss 6310, p 6310 (2020) |
| ISSN: | 1422-0067 |
| Popis: | The acceleration of peripheral nerve regeneration is crucial for functional nerve recovery. Our previous study demonstrated that human Wharton&rsquo s jelly-derived mesenchymal stem cells (hWJ-MSC) promote sciatic nerve recovery and regeneration via the direct upregulation and release of neurotrophic factors. However, the immunomodulatory role of hWJ-MSC in sciatic nerve recovery remains unclear. The effects of hWJ-MSC on innate immunity, represented by macrophages, natural killer cells, and dendritic cells, as well as on adaptive immunity, represented by CD4+ T, CD8+ T, B, and regulatory T cells (Tregs), were examined using flow cytometry. Interestingly, a significantly increased level of Tregs was detected in blood, lymph nodes (LNs), and nerve-infiltrating cells on POD7, 15, 21, and 35. Anti-inflammatory cytokines, such as IL-4 and IL-10, were significantly upregulated in the LNs and nerves of hWJ-MSC-treated mice. Treg depletion neutralized the improved effects of hWJ-MSC on sciatic nerve recovery. In contrast, Treg administration promoted the functional recovery of five-toe spread and gait stance. hWJ-MSC also expressed high levels of the anti-inflammatory cytokines TGF-&beta and IL-35. This study indicated that hWJ-MSC induce Treg development to modulate the balance between pro- and anti-inflammation at the injured sciatic nerve by secreting higher levels of anti-inflammatory cytokines. |
| Databáze: | OpenAIRE |
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